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        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2020-05-26</depositionDate>
            <releaseDate>2021-05-14</releaseDate>
            <updateDate>2021-08-17</updateDate>
        </keyDates>
        <title>3.9 Angstrom reconstruction of E.coli AcrB embedded in the liposome</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-9855-1641</authorORCID>
            <firstName>Xiao</firstName>
            <lastName>Fan</lastName>
            <organization type="academic">Department of Molecular Biology, Princeton University</organization>
            <street>Washington Rd.</street>
            <townOrCity>Princeton</townOrCity>
            <stateOrProvince>NJ</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>08544</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-4829-7416</authorORCID>
            <firstName>Nieng</firstName>
            <lastName>Yan</lastName>
            <organization type="academic">Department of Molecular Biology, Princeton University</organization>
            <street>Washington Rd.</street>
            <townOrCity>Princeton</townOrCity>
            <stateOrProvince>NJ</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>08544</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-2406-268X">Yao X</author>
            <author authorORCID="0000-0001-9855-1641">Fan X</author>
            <author authorORCID="0000-0003-4829-7416">Yan N</author>
        </authorsList>
        <datasetSize units="TB">9.6</datasetSize>
        <entryDOI>10.6019/EMPIAR-10426</entryDOI>
        <experimentType>EMDB</experimentType>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-22050</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
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                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0003-2406-268X" order="1">Yao X</author>
                    <author authorORCID="0000-0001-9855-1641" order="2">Fan X</author>
                    <author authorORCID="0000-0003-4829-7416" order="3">Yan N</author>
                    <title>Cryo-EM analysis of a membrane protein embedded in the liposome</title>
                    <journal>Proceedings of the National Academy of Sciences of the United States of America</journal>
                    <journalAbbreviation>Proc. Natl. Acad. Sci. U.S.A.</journalAbbreviation>
                    <country></country>
                    <year>2020</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1073/pnas.2009385117</externalReferences>
                    <externalReferences type="pubmed">32680969</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame micrographs of AcrB embedded in the liposome.</name>
        <directory>/data/movies</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRCS</headerFormat>
        <dataFormat>MRCS</dataFormat>
        <numImagesOrTiltSeries>5757</numImagesOrTiltSeries>
        <framesPerImage>32</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>7420</imageWidth>
            <pixelWidth>0.557</pixelWidth>
            <imageHeight>7676</imageHeight>
            <pixelHeight>0.557</pixelHeight>
        </dimensions>
        <details>Cs = 0.01mm. Total dose = 50 e-/A2. Particles are extracted with 2 fold binning (pix size 1.114 Angstrom).</details>
        <segmentationList/>
        <micrographsFilePattern>data/movies</micrographsFilePattern>
        <pickedParticlesFilePattern>data/refinement_particles.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/Polish/job100</pickedParticlesDirectory>
    </imageSet>
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