REL 2020-06-08 2021-11-08 2021-11-08 0000-0003-3550-6274 Mikhail Kudryashev Max Planck Institute of Biophysics Max-von-Laue-Strasse 3 Frankfurt am Main Hesse Germany 60438 0000-0003-3550-6274 Mikhail Kudryashev Max Planck Institute of Biophysics Max-von-Laue-Strasse 3 Frankfurt am Main Hesse Germany 60438 Dijkman PM Marzluf T Zhang Y Chang SYS Helm D Lanzer M Bujard H Kudryashev M 34.0 10.6019/EMPIAR-10437 EMDB EMD-11150 EMD-11151 EMD-11152 EMD-11153 EMD-11154 EMD-11155 EMD-11156 EMD-11157 Dijkman PM Marzluf T Zhang Y Chang SYS Helm D Lanzer M Bujard H Kudryashev M Science advances Sci Adv 23 7 2021 English 10.1126/sciadv.abg0465 34078606 Unaligned movies of MSP-1 /data/micrographs/MSP1 micrographs - multiframe MRC MRC 3734 40 1 40 32 BIT FLOAT 3838 1.073 3710 1.073

Unaligned, gain-corrected, 40-frame movies, recorded automatically with EPU, using K2 camera in counting mode on a Titan Krios microscope operated at 300 kV at a nominal magnification of 130 000, corresponding to a pixel size of 1.073 Å. The total electron dose per micrograph: ~64 e-/Å^2.

Unaligned movies of hdMSP-1 (dataset 1) /data/micrographs/hdMSP1-dataset1 micrographs - multiframe MRC MRC 1685 40 1 40 32 BIT FLOAT 3838 1.077 3710 1.077

Unaligned, gain-corrected, 40-frame movies, recorded automatically with EPU, using K2 camera in counting mode on a Titan Krios microscope operated at 300 kV at a nominal magnification of 130 000, corresponding to a pixel size of 1.077 Å. The total electron dose per micrograph: ~60 e-/Å^2. Datasets 1-4 were combined for reconstruction of hdMSP-1.

Unaligned movies of hdMSP-1 (dataset 2) /data/micrographs/hdMSP1-dataset2 micrographs - multiframe MRC MRC 3560 40 1 40 32 BIT FLOAT 3838 1.077 3710 1.077

Unaligned, gain-corrected, 40-frame movies, recorded automatically with EPU, using K2 camera in counting mode on a Titan Krios microscope operated at 300 kV at a nominal magnification of 130 000, corresponding to a pixel size of 1.077 Å. The total electron dose per micrograph: ~60 e-/Å^2. Datasets 1-4 were combined for reconstruction of hdMSP-1.

Unaligned movies of hdMSP-1 (dataset 3) /data/micrographs/hdMSP1-dataset3 micrographs - multiframe MRC MRC 5864 40 1 40 32 BIT FLOAT 3838 1.077 3710 1.077

Unaligned, gain-corrected, 40-frame movies, recorded automatically with EPU, using K2 camera in counting mode on a Titan Krios microscope operated at 300 kV at a nominal magnification of 130 000, corresponding to a pixel size of 1.077 Å. The total electron dose per micrograph: ~60 e-/Å^2. Datasets 1-4 were combined for reconstruction of hdMSP-1.

Unaligned movies of hdMSP-1 (dataset 4) /data/micrographs/hdMSP1-dataset4 micrographs - multiframe MRC MRC 1393 40 1 40 32 BIT FLOAT 3838 1.077 3710 1.077

Unaligned, gain-corrected, 40-frame movies, recorded automatically with EPU, using K2 camera in counting mode on a Titan Krios microscope operated at 300 kV at a nominal magnification of 130 000, corresponding to a pixel size of 1.077 Å. The total electron dose per micrograph: ~60 e-/Å^2. Datasets 1-4 were combined for reconstruction of hdMSP-1. Dataset 4 was collected at 15 degrees tilt.

Final particle stack for hdMSP-1 conformation 1 /data/particles/hdMSP1_conf1 picked particles - single frame - processed MRCS MRCS 271763 1 32 BIT FLOAT 288 1.077 288 1.077

Final 271,763 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for hdMSP-1 (conformation 1); box size 288 pixels, 1.077 Å/pixel.

Final particle stack for hdMSP-1 conformation 2 /data/particles/hdMSP1_conf2 picked particles - single frame - processed MRCS MRCS 182515 1 32 BIT FLOAT 288 1.077 288 1.077

Final 182,515 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for hdMSP-1 (conformation 2); box size 288 pixels, 1.077 Å/pixel.

Final particle stack for MSP-1 main conformation /data/particles/MSP1_mainfonf picked particles - single frame - processed MRCS MRCS 141838 1 32 BIT FLOAT 288 1.073 288 1.073

Final 141,838 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for MSP-1 (main conformation); box size 288 pixels, 1.073 Å/pixel.

Final particle stack for MSP-1 alternative conformation 1 /data/particles/MSP1_altconf1 picked particles - single frame - processed MRCS MRCS 112939 1 32 BIT FLOAT 288 1.073 288 1.073

Final 112,939 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for MSP-1 (alternative conformation 1); box size 288 pixels, 1.073 Å/pixel.

Final particle stack for MSP-1 alternative conformation 2 /data/particles/MSP1_altconf2 picked particles - single frame - processed MRCS MRCS 118878 1 32 BIT FLOAT 288 1.073 288 1.073

Final 118,878 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for MSP-1 (alternative conformation 2); box size 288 pixels, 1.073 Å/pixel.

Final particle stack for MSP-1 alternative conformation 3 /data/particles/MSP1_altconf3 picked particles - single frame - processed MRCS MRCS 124774 1 32 BIT FLOAT 288 1.073 288 1.073

Final 124,774 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for MSP-1 (alternative conformation 3); box size 288 pixels, 1.073 Å/pixel.

Final particle stack for MSP-1 alternative conformation 4 /data/particles/MSP1_altconf4 picked particles - single frame - processed MRCS MRCS 116037 1 32 BIT FLOAT 288 1.073 288 1.073

Final 116,037 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for MSP-1 (alternative conformation 4); box size 288 pixels, 1.073 Å/pixel.

Final particle stack for MSP-1 alternative conformation 5 /data/particles/MSP1_altconf5 picked particles - single frame - processed MRCS MRCS 75620 1 32 BIT FLOAT 288 1.073 288 1.073

Final 75,620 particle stack and corresponding starfile (converted from cryoSPARC particle file using USCF pyem) for MSP-1 (alternative conformation 5); box size 288 pixels, 1.073 Å/pixel.