REL
2020-06-10
2020-12-09
2021-01-29
Single particle cryo-EM dataset for MlaFEDB from E. coli in nanodisc
0000-0002-6050-2219
Nicolas
Coudray
NYU
540 1st Avenue, 2nd Floor Lab 14-17
New Yok
New York
United States
10016
0000-0002-2570-0404
Ekiert
Damian
NYU
540 1st Avenue, 2nd Floor Lab 14-17
New Yok
New York
United States
10016
0000-0003-0624-6178
Bhabha
Gira
NYU
540 1st Avenue, 2nd Floor Lab 14-17
New York
New York
United States
10016
Coudray N
Isom GL
MacRae M
Saiduddin MN
Bhabha G
Ekiert DC
5.2
10.6019/EMPIAR-10536
EMDB
EMD-22116
Coudray N
Isom GL
MacRae M
Saiduddin MN
Bhabha G
Ekiert DC
Structure of bacterial phospholipid transporter MlaFEDB with substrate bound
eLife
Elife
9
2020
English
10.7554/elife.62518
33236984
Raw movies acquired in super-resolution mode (0.416 A / pixel, 71 e-/A^2, 30 frames for 6 sec).
Particle stacks (0.832 A / pixel) were extracted from motion corrected movies (with Relion's implementation of motioncor2) followed by particle polishing.
Unaligned multi-frame micrographs of MlaFEDB complex
/data/movies
micrographs - multiframe
MRC
MRC
3212
30
UNSIGNED BYTE
7420
0.416
7676
0.416
Unaligned super-resolution (K2 camera, Titan Krios) multi-frame micrographs (total electron dose: 71 e-/A2; frame rate: 200 ms)
data/movies/18aug01*mrc
Polished particles (using Relion 3.1)
/data/polished_particles
picked particles - single frame - processed
MRCS
MRCS
3064
1
UNSIGNED BYTE
500
0.832
500
0.832
Picked particles after polishing in Relion 3.1.
data/polished_particles/18aug01*.mrcs
data/polished_particles/18aug01*frames_shiny.star
data/polished_particles