REL
2020-10-15
2022-06-22
2022-06-22
Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases
0000-0002-4414-5477
John
Kuriyan
Department of Molecular and Cell Biology and Department of Chemistry, University of California, Berkeley
176 Stanley Hall, MC 3220
Oakland
CA
United States
94720
0000-0002-4414-5477
John
Kuriyan
Department of Molecular and Cell Biology and Department of Chemistry, University of California, Berkeley
176 Stanley Hall, MC 3220
Oakland
CA
United States
94720
0000-0003-4231-4115
Sriram
Subramaniam
Djavad Mowafaghian Centre for Brain Health, The University of British Columbia
3404 – 2215 Wesbrook Mall
Vancouver
BC
Canada
V6T 1Z3
Kondo Y
Ognjenovic J
Banerjee S
Karandur D
Merk A
Kulhanek K
Wong K
Roose JP
Subramaniam S
Kuriyan J
6.2
10.6019/EMPIAR-10544
EMDB
EMD-20708
Kondo Y
Ognjenović J
Banerjee S
Karandur D
Merk A
Kulhanek K
Wong K
Roose JP
Subramaniam S
Kuriyan J
Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases
Science (New York, N.Y.)
Science
6461
366
109
115
2019
English
10.1126/science.aay0543
31604311
Human B-Raf kinase bound to Sf9 14-3-3 [multiple datasets in MRC and MRCS formats]
Unaligned multi-frame micrographs of B-Raf:14-3-3 complex
/data/Dataset1
micrographs - multiframe
MRCS
MRCS
1539
40
1
40
UNSIGNED BYTE
7420
0.532
7676
0.532
The micrographs are the unaligned, unnormalized super-resolution movie frames (40 frames per micrograph). Total accumulated dose is 50 electrons/Å^2 over a 12 seconds exposure. GainRef.mrc can be used with 270 degrees rotation.
Unaligned multi-frame micrographs of B-Raf:14-3-3 complex
/data/Dataset2
micrographs - multiframe
MRC
MRC
1451
40
1
40
UNSIGNED BYTE
7420
0.532
7676
0.532
The micrographs are the unaligned, unnormalized super-resolution movie frames (40 frames per micrograph). Total accumulated dose is 50 electrons/Å^2 over a 12 seconds exposure. GainRef.mrc requires no rotation.