Structure of the class D GPCR Ste2 dimer coupled to two G proteins

REL 2020-09-14 2020-12-04 2021-02-12 Structure of the class D GPCR Ste2 dimer coupled to two G proteins 0000-0001-8028-8486 Vaithish Velazhahan MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Cambridgeshire United Kingdom CB2 0QH 0000-0001-8028-8486 Vaithish Velazhahan MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Cambridgeshire United Kingdom CB2 0QH Velazhahan V Ma N Pándy-Szekeres G Kooistra AJ Lee Y Gloriam DE Vaidehi N Tate CG 6.4 10.6019/EMPIAR-10550 EMDB EMD-11720 Velazhahan V Ma N Pándy-Szekeres G Kooistra AJ Lee Y Gloriam DE Vaidehi N Tate CG Structure of the class D GPCR Ste2 dimer coupled to two G proteins Nature Nature United Kingdom 7840 589 148 153 2020 English 10.1038/s41586-020-2994-1 33268889 LMB Krios1 Movies /data/LMB_Krios1_Movies micrographs - multiframe MRC MRC 996 48 1 48 UNSIGNED BYTE 3838 1.145 3710 1.145

contains unaligned movies corresponding to the data collection "LMB Krios1". The real pixel size is 1.145 Å/pix, and the dose rate is 1.013 e/Å^2/frame. Please use data/LMB_Krios1_Movies/Micrographs/GainReference/FoilHole_16392868_Data_16385023_16385025_20191017_011206-gain-ref.MRC as gain reference without any rotation or flipping.

data/LMB_Krios1_Movies/Micrographs/*.mrc LMB Krios2 Movies /data/LMB_Krios2_Movies micrographs - multiframe TIFF TIFF 2027 40 1 40 UNSIGNED 16 BIT INTEGER 3838 1.1 3710 1.1

contains unaligned movies corresponding to the data collection "LMB Krios2". The real pixel size is 1.1 Å/pix, and the dose rate is 1.015 e/Å^2/frame. Please use data/LMB_Krios2_Movies/Micrographs/h1_s1/CountRef_krios2_24102019_h1_s1_i0001_movie_Oct24_17.44.14.mrc as gain reference without any rotation or flipping. The defects file can be found at data/LMB_Krios2_Movies/Micrographs/h1_s1/defects_krios2_24102019_h1_s1_i0001_movie_Oct24_17.44.14.txt. The "_hn_sn_" ,where n=1-9, portion of the file name refers to the hole and shot position within 3x3 image shift acquisition matrix (two non-overlapping shots per hole was acquired).

data/LMB_Krios2_Movies/Micrographs/h*_s*/*.tif eBic Krios1 Movies /data/eBIC_Krios1_Movies micrographs - multiframe MRC MRC 4637 45 1 45 UNSIGNED 16 BIT INTEGER 3838 1.047 3710 1.047

contains unaligned movies corresponding to the data collection "eBic Krios1". The real pixel size is 1.047 Å/pix, and the dose rate is 1.003 e/Å^2/frame. Please use data/eBIC_Krios1_Movies/Gain_reference/gain.mrc as gain reference without any rotation or flipping.

data/eBIC_Krios1_Movies/raw/GridSquare_276*****/Data/*.mrc