REL
2020-11-17
2022-02-04
2022-02-04
Nudaurelia capensis omega virus capsid: virus-like particles expressed in Nicotiana benthamiana
0000-0002-7637-4303
David
Mark
Lawson
John Innes Centre
Colney Lane
Norwich
Norfolk
United Kingdom
NR4 7UH
0000-0003-0700-906X
George
Peter
Lomonossoff
John Innes Centre
Colney Lane
Norwich
Norfolk
United Kingdom
NR4 7UH
Castells-Graells R
Ribeiro JRS
Domitrovic T
Hesketh EL
Scarff CA
Johnson JE
Ranson NA
Lawson DM
Lomonossoff GP
5.3
10.6019/EMPIAR-10560
EMDB
EMD-11830
Castells-Graells R
Ribeiro JRS
Domitrovic T
Hesketh EL
Scarff CA
Johnson JE
Ranson NA
Lawson DM
Lomonossoff GP
Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus
Communications biology
Commun Biol
1
4
2021
English
10.1038/s42003-021-02134-w
34031522
Data were collected on an FEI Titan Krios at 300 kV and recorded using the EPU automated acquisition software on a FEI Falcon III direct electron detector. Movies were acquired at a rate of 1.5 sec per movie with a dose of 79.5 e-/Å2 across 59 frames (1.35 e-/Å2 dose per frame), using a defocus range of -0.8 μm to -3.0 μm and a magnified pixel size of 1.0651 Å. Movie frames were aligned using MotionCor2 with 5 by 5 patches and B-factor of 150 Å2.
unaligned movies
/data/Images-Disc1
micrographs - multiframe
MRC
MRC
2788
59
1
59
UNSIGNED 16 BIT INTEGER
4096
1.097
4096
1.097
Micrographs are unaligned movies comprised of 59 frames each
data/motion_corrected_data/shift_eps_files - EPS files showing motion correction trajectories for each movie in the dataset.
data/motion_corrected_data/star_files - Star files from motion correction job.
data/Images-Disc1/GridSquare_*/Data/FoilHole_*_Fractions.mrc
aligned and dose-weighted micrographs
/data/motion_corrected_data/aligned_and_dose_weighted_micrographs
micrographs - single frame
MRC
MRC
2788
1
32 BIT FLOAT
4096
1.0651
4096
1.0651
Micrographs are averages of aligned and dose-weighted movies comprised of 59 frames
data/motion_corrected_data/shift_eps_files - EPS files showing motion correction trajectories for each movie in the dataset.
data/motion_corrected_data/star_files - Star files from motion correction job.
data/motion_corrected_data/aligned_and_dose_weighted_micrographs/FoilHole_*_Fractions.mrc