<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10665" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2020-06-17</depositionDate>
            <releaseDate>2022-03-28</releaseDate>
            <updateDate>2022-03-28</updateDate>
        </keyDates>
        <title>Single particle Cryo-EM data set for study the structural basis of Phosphatidylinositol 3-kinase type 2α (PI3KC2α)</title>
        <correspondingAuthor>
            <authorORCID>0000-0001-7904-6834</authorORCID>
            <firstName>Wen-Ting</firstName>
            <lastName>Lo</lastName>
            <organization type="academic">Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)</organization>
            <street>10 Robert-Rössle-Str</street>
            <townOrCity>Berlin</townOrCity>
            <country>Germany</country>
            <postOrZipCode>13125</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0003-3119-6993</authorORCID>
            <firstName>Volker</firstName>
            <lastName>Haucke</lastName>
            <organization type="academic">Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)</organization>
            <street>10 Robert-Rössle-Str</street>
            <townOrCity>Berlin</townOrCity>
            <country>Germany</country>
            <postOrZipCode>13125</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-7904-6834">Lo WT</author>
            <author authorORCID="0000-0002-6478-476X">Zhang Y</author>
            <author authorORCID="0000-0003-3511-6479">Vadas O</author>
            <author>Roske Y</author>
            <author authorORCID="0000-0001-7706-1635">Gulluni F</author>
            <author authorORCID="0000-0003-2785-0665">De Santis MC</author>
            <author>Zagar AV</author>
            <author>Stephanowitz H</author>
            <author authorORCID="0000-0002-9073-6024">Hirsch E</author>
            <author authorORCID="0000-0002-2358-549X">Liu F</author>
            <author authorORCID="0000-0002-6190-1414">Daumke O</author>
            <author authorORCID="0000-0003-3550-6274">Kudryashev M</author>
            <author authorORCID="0000-0003-3119-6993">Haucke V</author>
        </authorsList>
        <datasetSize units="TB">9.5</datasetSize>
        <entryDOI>10.6019/EMPIAR-10665</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-12191</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0001-7904-6834" order="1">Lo WT</author>
                    <author authorORCID="0000-0002-6478-476X" order="2">Zhang Y</author>
                    <author authorORCID="0000-0003-3511-6479" order="3">Vadas O</author>
                    <author order="4">Roske Y</author>
                    <author authorORCID="0000-0001-7706-1635" order="5">Gulluni F</author>
                    <author authorORCID="0000-0003-2785-0665" order="6">De Santis MC</author>
                    <author order="7">Zagar AV</author>
                    <author order="8">Stephanowitz H</author>
                    <author authorORCID="0000-0002-9073-6024" order="9">Hirsch E</author>
                    <author authorORCID="0000-0002-2358-549X" order="10">Liu F</author>
                    <author authorORCID="0000-0002-6190-1414" order="11">Daumke O</author>
                    <author authorORCID="0000-0003-3550-6274" order="12">Kudryashev M</author>
                    <author authorORCID="0000-0003-3119-6993" order="13">Haucke V</author>
                    <title>Structural basis of phosphatidylinositol 3-kinase C2α function</title>
                    <journal>Nature structural &amp; molecular biology</journal>
                    <journalAbbreviation>Nat Struct Mol Biol</journalAbbreviation>
                    <country></country>
                    <issue>3</issue>
                    <volume>29</volume>
                    <firstPage>218</firstPage>
                    <lastPage>228</lastPage>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41594-022-00730-w</externalReferences>
                    <externalReferences type="pubmed">35256802</externalReferences>
                    <details>The raw movies are collected with total 61 electrons per squared Angstrom (untilted) and 63 electrons per squared Angstrom 
(-30 degree tilted), over 50 frames.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>-30 degree tilted PI3KC2a data set raw movies</name>
        <directory>/data/30tilted_dataset</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>6568</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>50</frameRangeMax>
        </frameRange>
        <voxelType>SIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.837</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.837</pixelHeight>
        </dimensions>
        <details>The folder contains raw movies collected with stage -30 degree tilted, total dose is 60 electrons per squared Angstrom. Each movie has 50 frames.</details>
        <segmentationList/>
        <micrographsFilePattern>data/30tilted_dataset/FoilHole_13711097_Data_13711626_13711628_20200214_1217_fractions.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>untilted PI3KC2a data set raw movies</name>
        <directory>/data/PI3K_untilted</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>3865</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>50</frameRangeMax>
        </frameRange>
        <voxelType>SIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.837</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.837</pixelHeight>
        </dimensions>
        <details>The folder contains raw movies collected with stage untilted, total dose is about 60 electrons per squared Angstrom. Each movie has 50 frames.</details>
        <segmentationList/>
        <micrographsFilePattern>data/PI3K_untilted/FoilHole_32877137_Data_32877684_32877686_20191231_1406_fractions.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
