REL 2022-11-15 2023-08-18 2023-08-18 Cryo-EM structure of the wild-type solitary ECF module in MSP2N2 lipid nanodiscs in the ATPase open and nucleotide-free conformation (200 kV) 0000-0001-7017-109X Cristina Paulino Department of Biochemistry, Structural Biology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Nijenborgh 4 Groningen Netherlands 9747 AG 0000-0001-7017-109X Cristina Paulino Department of Biochemistry, Structural Biology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Nijenborgh 4 Groningen Netherlands 9747 AG 0000-0002-5804-9689 Dirk Jan Slotboom Faculty of Science and Engineering, Membrane Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Nijenborgh 4 Groningen Netherlands 9747 AG Thangaratnarajah C Rheinberger J Paulino C Slotboom DJ 1.9 10.6019/EMPIAR-11309 EMDB molecule EMD-16122 Thangaratnarajah C Nijland M Borges-Araújo L Jeucken A Rheinberger J Marrink SJ Souza PCT Paulino C Slotboom DJ Expulsion mechanism of the substrate-translocating subunit in ECF transporters Nature communications Nat Commun 1 14 2023 English 10.1038/s41467-023-40266-1 37491368 Unaligned movie frames (Dataset 1) /data/raw_210505_LdECF-MSP2N2-bCT47s3_ngc micrographs - multiframe TIFF TIFF 1400 60 UNSIGNED BYTE 3838 1.022 3710 1.022
Unaligned multi-frame movies with each containing 60 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 200 kV in counting mode. Total accumulated electron exposure on the specimen level corresponds to 50.1 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with SerialEM using a 3 x 3 multi-shot array.
 Unaligned movie frames (Dataset 2) /data/raw_210515_LdECF-MSP2N2-bCT48s2_ngc micrographs - multiframe TIFF TIFF 4205 60 UNSIGNED BYTE 3838 1.022 3710 1.022
Unaligned multi-frame movies with each containing 60 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 200 kV in counting mode. Total accumulated electron exposure on the specimen level corresponds to 50.1 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with SerialEM using a 3 x 3 multi-shot array.
 Unaligned movie frames (Dataset 3) /data/raw_210521_LdECF-MSP2N2-bCT48s4_ngc micrographs - multiframe TIFF TIFF 60 1401 UNSIGNED BYTE 3838 1.022 3710 1.022
Unaligned multi-frame movies with each containing 60 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 200 kV in counting mode. Total accumulated electron exposure on the specimen level corresponds to 50.1 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with SerialEM using a 3 x 3 multi-shot array.
 Unaligned movie frames (Dataset 4) /data/raw_210615_LdECF-MSP2N2-bCT49s2_ngc micrographs - multiframe TIFF TIFF 2981 60 UNSIGNED BYTE 3838 1.022 3710 1.022
Unaligned multi-frame movies with each containing 60 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 200 kV in counting mode. Total accumulated electron exposure on the specimen level corresponds to 50.1 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with SerialEM using a 3 x 3 multi-shot array.