REL 2022-11-15 2023-09-06 2023-09-06 Cryo-EM structure of the mutant solitary ECF module 2EQ in MSP2N2 lipid nanodiscs in the ATPase closed and ATP-bound conformation (300 kV) 0000-0001-7017-109X Cristina Paulino Department of Biochemistry, Structural Biology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Nijenborgh 4 Groningen Netherlands 9747 AG 0000-0001-7017-109X Cristina Paulino Department of Biochemistry, Structural Biology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Nijenborgh 4 Groningen Netherlands 9747 AG 0000-0002-5804-9689 Dirk Jan Slotboom Faculty of Science and Engineering, Membrane Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Nijenborgh 4 Groningen Netherlands 9747 AG Thangaratnarajah C Rheinberger J Paulino C Slotboom DJ 4.4 10.6019/EMPIAR-11311 EMDB molecule EMD-16124 Thangaratnarajah C Nijland M Borges-Araújo L Jeucken A Rheinberger J Marrink SJ Souza PCT Paulino C Slotboom DJ Expulsion mechanism of the substrate-translocating subunit in ECF transporters Nature communications Nat Commun 1 14 2023 English 10.1038/s41467-023-40266-1 37491368 Unaligned movie frames (Dataset 1) /data/raw_220226_LdECF-2EQv2-MSP2N2-bCT63s5_ngc micrographs - multiframe TIFF TIFF 9932 75 UNSIGNED BYTE 5760 0.836 4092 0.836
Unaligned multi-frame movies with each containing 75 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 300 kV in super-resolution counting mode with hardware binning by a factor of 2 (Counted Super Resolution Bin 2). Total accumulated electron exposure on the specimen level corresponds to 60 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with EPU and Aberration Free Image Shift (AFIS) enabled.
 Unaligned movie frames (Dataset 2) /data/raw_220228_LdECF-2EQv2-MSP2N2-bCT63s3_ngc micrographs - multiframe TIFF TIFF 5326 75 UNSIGNED BYTE 5760 0.836 4092 0.836
Unaligned multi-frame movies with each containing 75 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 300 kV in super-resolution counting mode with hardware binning by a factor of 2 (Counted Super Resolution Bin 2). Total accumulated electron exposure on the specimen level corresponds to 60 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with EPU and Aberration Free Image Shift (AFIS) enabled.