<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11321" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2022-11-22</depositionDate>
            <releaseDate>2023-03-14</releaseDate>
            <updateDate>2023-03-14</updateDate>
        </keyDates>
        <title>LRRC8A-BRIL(T48D):C Heteromer in GDN</title>
        <correspondingAuthor>
            <authorORCID>0000-0001-6768-3406</authorORCID>
            <firstName>Stephen</firstName>
            <middleName>G</middleName>
            <lastName>Brohawn</lastName>
            <organization type="academic">UC Berkeley</organization>
            <street>Weill Hall</street>
            <townOrCity>Berkeley</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94720</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0001-8529-9045</authorORCID>
            <firstName>David</firstName>
            <middleName>M</middleName>
            <lastName>Kern</lastName>
            <organization type="academic">UC Berkeley</organization>
            <street>Weill Hall</street>
            <townOrCity>Berkeley</townOrCity>
            <stateOrProvince>CA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94720</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator>
            <authorORCID>0000-0001-6768-3406</authorORCID>
            <firstName>Stephen</firstName>
            <middleName>G</middleName>
            <lastName>Brohawn</lastName>
            <organization type="academic">UC Berkeley</organization>
            <street>Weill Hall</street>
            <townOrCity>Berkeley</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94720</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-8529-9045">Kern DM</author>
            <author authorORCID="0000-0001-6768-3406">Brohawn SG</author>
        </authorsList>
        <datasetSize units="TB">2.1</datasetSize>
        <entryDOI>10.6019/EMPIAR-11321</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-28898</emdbEntry>
            <emdbEntry>EMD-28901</emdbEntry>
            <emdbEntry>EMD-28903</emdbEntry>
            <emdbEntry>EMD-28904</emdbEntry>
            <emdbEntry>EMD-28905</emdbEntry>
            <emdbEntry>EMD-28897</emdbEntry>
            <emdbEntry>EMD-28894</emdbEntry>
            <emdbEntry>EMD-28895</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="true">
                    <author authorORCID="0000-0001-8529-9045" order="1">Kern DM</author>
                    <author authorORCID="0000-0001-9146-9864" order="2">Bleier J</author>
                    <author order="3">Mukherjee S</author>
                    <author order="4">Hill JM</author>
                    <author order="5">Kossiakoff AA</author>
                    <author authorORCID="0000-0003-4775-9359" order="6">Isacoff EY</author>
                    <author authorORCID="0000-0001-6768-3406" order="7">Brohawn SG</author>
                    <title>Structural basis for assembly and lipid-mediated gating of LRRC8A:C volume-regulated anion channels</title>
                    <journal>bioRxiv</journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1101/2022.07.31.502239</externalReferences>
                    <externalReferences type="pubmed">30775971</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned tif movies for LRRC8A-BRIL(T48D):C in GDN</name>
        <directory>/data/Frames</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>6939</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>1.048</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>1.048</pixelHeight>
        </dimensions>
        <details>Gain reference in mrc form is included in the folder with the movies. In RELION, the gain must be flipped upside down. To match our motion correction settings, use 0.9957 e/Å2 dose per frame and do not bin during motion correction.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Polished particles stacks for LRRC8A-BRIL(T48D)/C in GDN</name>
        <directory>/data/EMPIARparticles/PolishedParticles</directory>
        <category>picked particles - multiframe - processed</category>
        <headerFormat>MRCS</headerFormat>
        <dataFormat>MRCS</dataFormat>
        <numImagesOrTiltSeries>5988</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>416</imageWidth>
            <pixelWidth>1.048</pixelWidth>
            <imageHeight>416</imageHeight>
            <pixelHeight>1.048</pixelHeight>
        </dimensions>
        <details>Contains polished particles (145,915) as 5988 mrcs files. The selections of particles for each conformation (1 and 2) are in the EMPIARparticles folder. They are in RELION star format and can be imported into cryoSPARC as well for processing.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
