REL 2022-11-22 2024-02-01 2024-02-01 0000-0001-9226-3382 Romain Linares Institut de Biologie Structurale 71 avenue des martyrs GRENOBLE - France 38044 CEDEX9 0000-0001-9226-3382 Romain Linares Institut de Biologie Structurale 71 avenue des Martyrs GRENOBLE France 38044 CEDEX 9 Linares R Arnaud C Effantin G Darnault C Epalle NH Erba EB Schoehn G Breyton C 3.0 10.6019/EMPIAR-11327 EMDB virus EMD-13953 EMD-14799 EMD-14800 EMD-14873 Linares R Arnaud C Effantin G Darnault C Epalle NH Erba EB Schoehn G Breyton C bioRxiv 2022 10.1101/2022.09.20.507954

This is a cryo-EM sample of purified tails from bacteriophage T5, after incubation with E.coli bacterial receptor FhuA inserted into nanodiscs. This is the second of two separate data collections done on this sample. Typically, 3.5 µL of T5 tail incubated with FhuA-nanodisc sample were deposited on a freshly glow discharged (25 mA, 30 sec) Cu/Rh 300 mesh Quantifoil R 2/1 EM grids and plunge frozen in nitrogen-cooled liquid ethane using a ThermoFisher Mark IV Vitrobot device (100% humidity, 20°C, 5s blotting time, blot force 0) The dataset contains 5733 40-frame movie, acquired on a Thermo Fisher Scientific Titan Krios G3 TEM (European Synchrotron Radiation Facility, Grenoble, France) operated at 300 kV and equipped with a Gatan Quantum energy filter coupled to a Gatan K2 summit direct electron detector. Automated data collection was performed using Thermo Fisher Scientific EPU software, with a typical defocus range of -1.0 to -3.0 µm and a total dose of 40 e-/Å2 per movie. A nominal magnification of 105.000x was used, resulting in a calibrated pixel size at the specimen level of 1.351 Å.

Linares R Arnaud CA Effantin G Darnault C Epalle NH Boeri Erba E Schoehn G Breyton C Science advances Sci Adv 12 9 2023 English 10.1126/sciadv.ade9674 36961893 Unaligned multi-frame micrographs of phage T5 tails pre-incubated with bacteria receptor FhuA inserted in nanodiscs /data micrographs - multiframe MRC MRC 5733 40 UNSIGNED BYTE 3838 1.351 3710 1.351