REL
2022-11-30
2023-01-31
2023-01-31
Cryo-EM structure of a delivery complex containing the SspB adaptor, an ssrA-tagged substrate, and the AAA+ ClpXP protease
0000-0002-1719-5399
Robert
Sauer
MIT
77 Massachusetts Ave.
Cambridge
MA
United States
02139
0000-0002-8858-8907
Joseph
H
Davis
MIT
77 Massachusetts Ave.
Cambridge
MA
United States
02139
Ghanbarpour A
Fei X
Baker TA
Davis JH
Sauer RT
4.0
10.6019/EMPIAR-11349
EMDB
molecule
EMD-28585
Ghanbarpour A
Fei X
Baker TA
Davis JH
Sauer RT
The SspB adaptor drives structural changes in the AAA<sup>+</sup>ClpXP protease during ssrA-tagged substrate delivery
bioRxiv
2022
English
10.1101/2022.11.06.515074
Unaligned Movies of a Delivery Complex Containing the SspB Adaptor, an ssrA-Tagged Substrate, and the AAA+ ClpXP Protease
/data
micrographs - multiframe
TIFF
TIFF
9524
40
UNSIGNED BYTE
11520
0.435
8184
0.435
Nominal Defocus range (min, max, step): -1.0, -2.5, -0.25
Total exposure on specimen: 75.98 el/A2
Exposure time: 2.02 s
Nominal pixel size: 0.87 A (0.435 A super res)
Calibrated pixel size: 0.832 (0.416 A super res)
Objective lens spherical aberration (Cs): 2.7 mm
Acceleration voltage: 300 kV
Extraction voltage: 3550 V
Microscope / Detector: Krios G3i / K3