REL 2022-11-03 2023-01-30 2023-05-12 Single particle cryo-EM dataset for E. coli MlaFEDB bound to MlaC 0000-0003-0624-6178 Gira Bhabha NYU N/A New York New York United States 10016 0000-0003-0624-6178 Gira Bhabha NYU New York New York United States 10016 0000-0002-2570-0404 Damian C. Ekiert NYU New York New York United States 10016 MacRae MR Coudray N Bhabha G Ekiert DC 5.0 10.6019/EMPIAR-11354 EMDB molecule EMD-40162 MacRae MR Puvanendran D Haase MAB Coudray N Kolich L Lam C Baek M Bhabha G Ekiert DC Protein-protein interactions in the Mla lipid transport system probed by computational structure prediction and deep mutational scanning The Journal of biological chemistry J Biol Chem 2023 English 10.1016/j.jbc.2023.104744 37100290
Un-tilted and tilted movies were acquired via Leginon (Cheng et al., 2021; Suloway et al., 2005) on an Arctica microscope at 200 kV equipped with a Gatan K3 camera, at 0.548 Å/pixel in super-resolution mode.
 MacRae MR Puvanendran D Haase MAB Coudray N Kolich L Lam C Minkyung B Bhabha G Protein-protein interactions in the Mla lipid transport system probed by computational structure prediction and deep mutational scanning bioRxiv 2022 10.1101/2022.12.09.519820
Un-tilted and tilted movies were acquired via Leginon (Cheng et al., 2021; Suloway et al., 2005) on an Arctica microscope at 200 kV equipped with a Gatan K3 camera, at 0.548 Å/pixel in super-resolution mode.
 Aligned frames of MlaFEDB & MlaC complex (tilted dataset) /data/tilted/aligned micrographs - single frame MRC MRC 3874 1 32 BIT FLOAT 5760 1.096 4092 1.096
Aligned micrographs (tilted dataset)
data/tilted/aligned/*-b-DW.mrc unaligned frames of MlaFEDB & MlaC complex (tilted dataset) /data/tilted/frames micrographs - multiframe TIFF TIFF 3874 48 1 48 UNSIGNED BYTE 11520 0.548 8184 0.548
Unaligned frames (tilted dataset)
data/tilted/frames/*.tif Aligned frames of MlaFEDB & MlaC complex (untilted dataset) /data/untilted micrographs - single frame MRC MRC 3449 1 32 BIT FLOAT 5760 1.096 4092 1.096
Aligned micrographs (untilted dataset)
data/untilted/*/aligned/*-a-DW.mrc Unaligned frames of MlaFEDB & MlaC complex (untilted dataset) /data/untilted micrographs - multiframe TIFF TIFF 3449 40 1 40 UNSIGNED BYTE 11520 0.548 8184 0.548
Unaligned frames (untilted dataset)
data/untilted/*/frames/*.frames.tif