REL 2023-01-09 2023-01-25 2023-01-25 0000-0003-1332-6002 Kimon Lemonidis School of Molecular Biosciences, College of Medical, Veterinary & Life Sciences, University of Glasgow Room 309, Davidson building Glasgow Scotland United Kingdom G12 8QQ 0000-0002-4289-4810 Helen Walden School of Molecular Biosciences, College of Medical, Veterinary & Life Sciences, University of Glasgow Room 332, Davidson building Glasgow Scotland United Kingdom G12 8QQ Lemonidis K Rennie ML Arkinson C Chaugule VK Clarke M Streetley J Walden H 2.9 10.6019/EMPIAR-11372 EMDB molecule EMD-14694 Lemonidis K Rennie ML Arkinson C Chaugule VK Clarke M Streetley J Walden H The EMBO journal EMBO J United Kingdom 2022 English 10.15252/embj.2022111898 36385258 Unaligned multi-frame movies of ubiquitinated FANCI in complex with FANCD2 and double-stranded DNA /data/dataset_1 micrographs - multiframe TIFF TIFF 5414 45 32 BIT FLOAT 4096 1.023 4096 1.023

Imaging was performed on JEOL CRYO ARM 300 microscope, equipped with a DE64 detector (Magnification: 120,000x, acceleration voltage: 300 kV, spherical aberration: 2.7 mm, electron exposure: 45.2 e/Å^2). Movies were recorded in MRC format and were subsequently LZW compressed in TIFF format using RELION. For CryoSPARC processing, the corresponding gain reference file needs to be flipped in both Y and X axes.

Unaligned multi-frame movies of ubiquitinated FANCI in complex with FANCD2 and double-stranded DNA (energy filtered imaging) /data/dataset_2 micrographs - multiframe TIFF TIFF 5814 45 32 BIT FLOAT 4096 1.023 4096 1.023

Imaging was performed on JEOL CRYO ARM 300 microscope, equipped with a DE64 detector and an in-column omega filter (Magnification: 120,000x, acceleration voltage: 300 kV, spherical aberration: 2.7 mm, electron exposure: 46.8 e/Å^2, slit width: 30 eV). Movies were recorded in MRC format and were subsequently LZW compressed in TIFF format using RELION. For CryoSPARC processing, the corresponding gain reference file needs to be flipped in Y axis.