REL 2023-01-25 2023-04-11 2023-04-11 0000-0003-4738-9503 Max Wilkinson Broad Institute of MIT and Harvard 75 Ames St Cambridge Massachusetts United States 02142 0000-0003-0178-7995 Feng Zhang Broad Institute of MIT and Harvard Cambridge United States 02142 Wilkinson ME Zhang F Howard Hughes Medical Institute (HHMI) NA United States National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) R01HG009761 United States 2.3 10.6019/EMPIAR-11458 EMDB molecule EMD-40033 Wilkinson ME Frangieh CJ Macrae RK Zhang F Science (New York, N.Y.) Science 2023 English 10.1126/science.adg7883 37023171 Unaligned multi-frame movies of Bombyx mori R2 retrotransposon initiating target-primed reverse transcription /data/Movies/Images-Disc1 micrographs - multiframe TIFF TIFF 16551 40 1 40 UNSIGNED BYTE 5760 0.663 4092 0.663

This entry contains 16551 K3 movies in compressed tiff format Collection info: Titan Krios G3i Voltage: 300 keV Cs: 2.7 mm Gatan K3 detector 130000x magnification 0.663 A/pix 20 eV energy filter 100 uM objective aperture 42.2 e/A2 over 40 frames Gain reference: K3-GainRef-bin2.mrc For RELION, use gain rotation 0 (no rotation) and gain flip 1 (flip upside down) refined_particles_data.star is the run_data.star file for the final refinement Note: many of the particles did not align well, so the final refinement used a very small fraction of the full data set. You might have better luck though! Sample: Bombyx mori R2 retrotransposon initiating target-primed reverse transcription Grid: Quantifoil Cu 300 mesh R2/2 + 2 nm carbon Cemera: Gatan K3 post a BioQuantum energy filter Camera mode: super resolution, 2-fold binning by EPU TEM mode: EFTEM NanoProbe Mag: x130k C2 aperture: 50 um Spot: 4 Beam diameter: 0.75 um EF slit: 20 eV Data collection software: EPU Acquisition method: AFIS Acquisition pattern: 9 images per hole, 12 um distance per stage shift Physical Pixel: 0.663 A/pix SuperRes pixel: 0.3315 A/pix Exposure rate: 26.9 e/pix/s Exposure time: 0.69 s Total exposure: 42.2 e/A2 Frames: 40 Defocus: 1.5 - 2.5 um Movies: 16551 Data format: non-gain-normalized LZW compressed TIFFs Gain reference: "K3-GainRef-bin2.mrc" Final particles: 39616 Particles file: "refined_particles_data.star"

data/Movies/Images-Disc1/GridSquare_*/Data/FoilHole_*_fractions.tiff data/Movies/refined_particles_data.star