<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11481" schemaVersion="0.63" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2023-02-08</depositionDate>
            <releaseDate>2023-08-18</releaseDate>
            <updateDate>2023-08-18</updateDate>
        </keyDates>
        <title>Movies of GroEL-ES-nucleotide complex plunged 13, 50, 200 ms and 20 s after mixing with ATP</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-7516-8658</authorORCID>
            <firstName>Rouslan</firstName>
            <middleName>G</middleName>
            <lastName>Efremov</lastName>
            <organization type="academic">vrije universiteit Brussels</organization>
            <street>pleinlaan 2</street>
            <townOrCity>Brussels</townOrCity>
            <stateOrProvince>Brussel</stateOrProvince>
            <country>Belgium</country>
            <postOrZipCode>1050</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-7516-8658</authorORCID>
            <firstName>Rouslan</firstName>
            <middleName>G</middleName>
            <lastName>Efremov</lastName>
            <organization type="academic">vrije universiteit Brussels</organization>
            <street>pleinlaan 2</street>
            <townOrCity>Brussels</townOrCity>
            <stateOrProvince>Brussel</stateOrProvince>
            <country>Belgium</country>
            <postOrZipCode>1050</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-7511-3507">Torino S</author>
            <author authorORCID="0000-0002-4342-0764">Dhurandhar M</author>
            <author authorORCID="0000-0002-8888-583X">Stroobants A</author>
            <author authorORCID="0000-0002-5157-0359">Claessens R</author>
            <author authorORCID="0000-0001-7516-8658">Efremov RG</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>European Research Council (ERC)</fundingBody>
                <code>726436</code>
                <country>Belgium</country>
            </grantReference>
            <grantReference>
                <fundingBody>Research Foundation - Flanders (FWO)</fundingBody>
                <code>G0H5916N</code>
                <country>Belgium</country>
            </grantReference>
            <grantReference>
                <fundingBody>Research Foundation - Flanders (FWO)</fundingBody>
                <code>G054617N</code>
                <country>Belgium</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">2.9</datasetSize>
        <entryDOI>10.6019/EMPIAR-11481</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-16102</emdbEntry>
            <emdbEntry>EMD-16107</emdbEntry>
            <emdbEntry>EMD-16115</emdbEntry>
            <emdbEntry>EMD-16108</emdbEntry>
            <emdbEntry>EMD-16106</emdbEntry>
            <emdbEntry>EMD-16109</emdbEntry>
            <emdbEntry>EMD-16100</emdbEntry>
            <emdbEntry>EMD-16116</emdbEntry>
            <emdbEntry>EMD-16125</emdbEntry>
            <emdbEntry>EMD-16118</emdbEntry>
            <emdbEntry>EMD-16117</emdbEntry>
            <emdbEntry>EMD-16119</emdbEntry>
            <emdbEntry>EMD-16099</emdbEntry>
            <emdbEntry>EMD-16154</emdbEntry>
            <emdbEntry>EMD-16157</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="true">
                    <author authorORCID="0000-0002-7511-3507" order="1">Torino S</author>
                    <author authorORCID="0000-0002-4342-0764" order="2">Dhurandhar M</author>
                    <author authorORCID="0000-0002-8888-583X" order="3">Stroobants A</author>
                    <author authorORCID="0000-0002-5157-0359" order="4">Claessens R</author>
                    <author authorORCID="0000-0001-7516-8658" order="5">Efremov RG</author>
                    <title>Time-resolved cryo-EM using a combination of droplet microfluidics with on-demand jetting</title>
                    <journal>Nature Methods</journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country>Belgium</country>
                    <externalReferences type="doi">10.1038/s41592-023-01967-z</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>GroELES with 20s of mixing time</name>
        <directory>/data/20s</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>4194</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>variable</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>variable</pixelHeight>
        </dimensions>
        <details>STAR files associated with the reconstructed maps from this dataset are uploaded in the directory 'associated_star_files' with names 20sGroEL-ATP.star, 20sGroEL-ATP-GroES.star, 20sGroEL-ATP-ADP-GroES.star and 20s_GroEL-ATP-2GroES.star

gain files are included in the Gain file directory under particular time point. Please use the corresponding gain file by matching the gain file date and micrograph date. 
The dates are in format YYYYMMDD
The date is indicated at the start of micrograph name e.g 20220628_trGroEL...tif
The date is the second part of gain refence file name e.g CountRef_20220628_trGroEL...mrc</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>GroELES at 50ms timepoint</name>
        <directory>/data/50ms</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>1416</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>variable</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>variable</pixelHeight>
        </dimensions>
        <details>STAR files associated with the reconstructed maps from this dataset are uploaded in the directory 'associated_star_files' with names 50ms_GroEL-ADP.star, 50ms_GroEL-ATP-ADP.star, 50ms_GroEL-ATP.star

gain files are included in the Gain file directory under particular time point. Please use the corresponding gain file by matching the gain file date and micrograph date. 
The dates are in format YYYYMMDD
The date is indicated at the start of micrograph name e.g 20220628_trGroEL...tif
The date is the second part of gain refence file name e.g CountRef_20220628_trGroEL...mrcGroEL was mixed with GroES and ATP and plunged within 50ms</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>GroEL 13ms timepoint</name>
        <directory>/data/10ms</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>2609</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>variable</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>variable</pixelHeight>
        </dimensions>
        <details>STAR files associated with the reconstructed maps from this dataset are uploaded in the directory 'associated_star_files' with names 13ms_GroEL-ADP.star, 13ms-GroEL-ATP-ADP.star, 13ms_GroEL-ATP.star

gain files are included in the Gain file directory under particular time point. Please use the corresponding gain file by matching the gain file date and micrograph date. 
The dates are in format YYYYMMDD
The date is indicated at the start of micrograph name e.g 20220628_trGroEL...tif
The date is the second part of gain refence file name e.g CountRef_20220628_trGroEL...mrc</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>GroELES 200ms timepoint</name>
        <directory>/data/200ms</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>5445</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>variable</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>variable</pixelHeight>
        </dimensions>
        <details>STAR files associated with the reconstructed maps from this dataset are uploaded in the directory 'associated_star_files' with names 200msGroEL-ATP.star, 200msGroEL-ATP-GroES.star, 200msGroEL-ATP-ADP-GroES.star and 200ms_GroEL-ATP-2GroES.star

gain files are included in the Gain file directory under particular time point. Please use the corresponding gain file by matching the gain file date and micrograph date. 
The dates are in format YYYYMMDD
The date is indicated at the start of micrograph name e.g 20220628_trGroEL...tif
The date is the second part of gain refence file name e.g CountRef_20220628_trGroEL...mrc</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
