REL
2023-03-01
2023-11-07
2023-11-07
Single particle cryo-EM dataset of extended cowpea chlorotic mottle virus in plunge-frozen vitrified ice
0000-0001-7319-6268
Sarah
Victoria
Barrass
Laboratory of Molecular Nanodynmaics, Ecole Polytechnique Fédérale de Lausanne
CH G0 615 (Bât. CH)
Lausanne
Vaud
Switzerland
CH-1015
0000-0002-8869-5999
Ulrich
Lorenz
Laboratory of Molecular Nanodynmaics, Ecole Polytechnique Fédérale de Lausanne
CH G0 615 (Bât. CH)
Lausanne
Vaud
Switzerland
CH-1015
Harder OF
Barrass SV
Drabbels M
Lorenz UJ
Swiss Nanoscience Institute
PP00P2_163681
Switzerland
European Research Council (ERC)
759145
Switzerland
5.1
10.6019/EMPIAR-11487
EMDB
virus
EMD-16790
Harder OF
Barrass SV
Drabbels M
Lorenz UJ
Fast viral dynamics revealed by microsecond time-resolved cryo-EM
Nature communications
Nat Commun
1
14
2023
English
10.1038/s41467-023-41444-x
37704664
Single particle cryo-EM dataset of extended cowpea chlorotic mottle virus in plunge-frozen vitrified ice, grid 1
/data/Images-Disc1/set1
micrographs - multiframe
EER
EER
5391
1505
BIT
4096
0.726
4096
0.726
These are the EER files from Falcon 4 detector - raw micrographs, not motion corrected.
Samples of expanded CCMV are prepared at pH 7.6 in the absence of divalent ions and plunge frozen in the presence of a photoacid (NPE-caged proton). The pH of the sample is then lowered to 4.5 by releasing the photoacid with UV irradiation (266 nm).
Single particle cryo-EM dataset of extended cowpea chlorotic mottle virus in plunge-frozen vitrified ice, grid 2
/data/Images-Disc1/set2
micrographs - multiframe
EER
EER
5235
1428
BIT
4096
0.726
4096
0.726
These are the EER files from Falcon 4 detector - raw micrographs, not motion corrected.
Samples of expanded CCMV are prepared at pH 7.6 in the absence of divalent ions and plunge frozen in the presence of a photoacid (NPE-caged proton). The pH of the sample is then lowered to 4.5 by releasing the photoacid with UV irradiation (266 nm).