REL
2023-04-07
2023-04-25
2023-04-25
Cryo-EM structure of pre-consensus elemental paused elongation complex
0000-0002-8241-3153
Seth
A
Darst
The Rockefeller University
1230 York Avenue
New York
NY
United States
10065
0000-0002-8241-3153
Seth
A
Darst
The Rockefeller University
1230 York Avenue
New York
NY
United States
10065
0000-0002-5042-0383
Robert
Landick
Department of Biochemistry, University of Wisconsin-Madison
Madison
WI
United States
53706
Kang JY
Mishanina TV
Bao Y
Chen J
Llewellyn E
Liu J
Darst SA
Landick R
Burroughs Wellcome Fund
CASI award 1016945
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R35 GM118130
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01 GM38330
United States
1.9
10.6019/EMPIAR-11506
EMDB
molecule
EMD-28109
Kang JY
Mishanina TV
Bao Y
Chen J
Llewellyn E
Liu J
Darst SA
Landick R
An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options
Proceedings of the National Academy of Sciences of the United States of America
Proc Natl Acad Sci U S A
8
120
2023
English
10.1073/pnas.2215945120
36795753
Grids were imaged using a 300keV Titan Krios (ThermoFisher Scientific) equipped with a K2 Summit direct electron detector (Gatan, Pleasanton, CA). Images were recorded with Serial EM in super-resolution mode with a super-resolution pixel size of 0.65 A and a defocus range of -0.8 to -2.4 micron. Data were collected with a dose rate of 8 electrons/physical pixel/s (1.3 A pixel size at the speciment). Images were recorded with a 10 s exposure and 0.2 s sub-frames (50 total frames) to give a total dose of 47.34 electrons/A2.
Unaligned multi-frame micrographs of pre-consensus elemental paused elongation complex
/data
micrographs - multiframe
TIFF
TIFF
4419
50
UNSIGNED BYTE
7420
0.6625
7676
0.6625