REL 2023-07-03 2023-11-14 2023-11-14 0000-0001-7017-109X Cristina Paulino University of Heidelberg, Biochemistry Center (BZH) Im Neuenheimer Feld 328 Heidelberg Germany 69120 0000-0001-7017-109X Cristina Paulino University of Heidelberg, Biochemistry Center (BZH) Im Neuenheimer Feld 328 Heidelberg Germany 69120 Kalienkova V Peter MF Rheinberger J Paulino C Netherlands Organisation for Scientific Research (NWO) 722.017.001 Netherlands Netherlands Organisation for Scientific Research (NWO) 40.018.016 Netherlands Swiss National Science Foundation P2ZHP3_187679 Switzerland Swiss National Science Foundation P500PB_203053 Switzerland 3.3 10.6019/EMPIAR-11628 EMDB molecule EMD-17596 EMD-17598 EMD-17599 EMD-17601 EMD-17602 Kalienkova V Peter MF Rheinberger J Paulino C Nature Nature 7985 623 202 209 2023 English 10.1038/s41586-023-06629-w 37880361 Unaligned movie frames of ligand-free SpSLC9C1 in lipid nanodiscs /data/raw_220117_spSLC9C1-2N2-apo-bVK11s2_ngc micrographs - multiframe TIFF TIFF 11299 76 UNSIGNED BYTE 5760 0.836 4092 0.836

Unaligned multi-frame movies with each containing 76 frames per movie collected at 300 kV in super-resolution counting mode with hardware binning by a factor of 2 (Counted Super Resolution Bin 2). Total accumulated electron exposure on the specimen level corresponds to 60 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with EPU, recording 2 images / hole, with Aberration Free Image Shift (AFIS) enabled.