<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11635" schemaVersion="0.63" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2023-07-27</depositionDate>
            <releaseDate>2023-12-01</releaseDate>
            <updateDate>2023-12-01</updateDate>
        </keyDates>
        <title>cAMP-bound SpSLC9C1 in lipid nanodiscs</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-7017-109X</authorORCID>
            <firstName>Cristina</firstName>
            <lastName>Paulino</lastName>
            <organization type="academic">University of Heidelberg, Biochemistry Center (BZH)</organization>
            <street>Im Neuenheimer Feld 328</street>
            <townOrCity>Heidelberg</townOrCity>
            <country>Germany</country>
            <postOrZipCode>69120</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-7017-109X</authorORCID>
            <firstName>Cristina</firstName>
            <lastName>Paulino</lastName>
            <organization type="academic">University of Heidelberg, Biochemistry Center (BZH)</organization>
            <street>Im Neuenheimer Feld 328</street>
            <townOrCity>Heidelberg</townOrCity>
            <country>Germany</country>
            <postOrZipCode>69120</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-4143-6172">Kalienkova V</author>
            <author authorORCID="0000-0001-6549-8127">Peter MF</author>
            <author authorORCID="0000-0002-9901-2065">Rheinberger J</author>
            <author authorORCID="0000-0001-7017-109X">Paulino C</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Netherlands Organisation for Scientific Research (NWO)</fundingBody>
                <code>722.017.001</code>
                <country>Netherlands</country>
            </grantReference>
            <grantReference>
                <fundingBody>Netherlands Organisation for Scientific Research (NWO)</fundingBody>
                <code>40.018.016</code>
                <country>Netherlands</country>
            </grantReference>
            <grantReference>
                <fundingBody>Swiss National Science Foundation</fundingBody>
                <code>P2ZHP3_187679</code>
                <country>Switzerland</country>
            </grantReference>
            <grantReference>
                <fundingBody>Swiss National Science Foundation</fundingBody>
                <code>P500PB_203053</code>
                <country>Switzerland</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">4.8</datasetSize>
        <entryDOI>10.6019/EMPIAR-11635</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-17605</emdbEntry>
            <emdbEntry>EMD-17607</emdbEntry>
            <emdbEntry>EMD-17625</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="false">
                    <author authorORCID="0000-0002-4143-6172" order="1">Kalienkova V</author>
                    <author authorORCID="0000-0001-6549-8127" order="2">Peter MF</author>
                    <author authorORCID="0000-0002-9901-2065" order="3">Rheinberger J</author>
                    <author authorORCID="0000-0001-7017-109X" order="4">Paulino C</author>
                    <title>Structures of a sperm-specific solute carrier gated by voltage and cAMP</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned movie frames of cAMP-bound SpSLC9C1 in nanodiscs, dataset 1</name>
        <directory>/data/raw_220810_spSLC9C1-2N2-cAMP-bVK24s1_ngc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>1706</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.022</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.022</pixelHeight>
        </dimensions>
        <details>Unaligned movie frames (60 frames per movie), collected at 200 kV in counting mode. Total electron dose is 53.599 electrons/Å^2. Data were recorded with SerialEM using 3 x 3 multi-shot pattern. Movies are not gain-corrected, gain reference is provided.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned movie frames of cAMP-bound SpSLC9C1 in nanodiscs, dataset 2</name>
        <directory>/data/raw_220810_spSLC9C1-2N2-cAMP-bVK24s2_ngc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>159</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.022</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.022</pixelHeight>
        </dimensions>
        <details>Unaligned movie frames (60 frames per movie), collected at 200 kV in counting mode. Total electron dose is 53.599 electrons/Å^2. Data were recorded with SerialEM using 3 x 3 multi-shot pattern. Movies are not gain-corrected, gain reference is provided.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned movie frames of cAMP-bound SpSLC9C1 in nanodiscs, dataset 3</name>
        <directory>/data/raw_220819_spSLC9C1-2N2-cAMP-bVK23s6_merged_ngc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>818</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.022</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.022</pixelHeight>
        </dimensions>
        <details>Unaligned movie frames (60 frames per movie), collected at 200 kV in counting mode. Total electron dose is 53.599 electrons/Å^2. Data were recorded with SerialEM using 3 x 3 multi-shot pattern. Movies are not gain-corrected, gain reference is provided.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned movie frames of cAMP-bound SpSLC9C1 in nanodiscs, dataset 4</name>
        <directory>/data/raw_220908_spSLC9C1-2N2-cAMP-FF8-bVK25s1_ngc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>3742</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.022</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.022</pixelHeight>
        </dimensions>
        <details>Unaligned movie frames (60 frames per movie), collected at 200 kV in counting mode. Total electron dose is 49.95 electrons/Å^2. Data were recorded with SerialEM using 3 x 3 multi-shot pattern. Movies are not gain-corrected, gain reference is provided.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned movie frames of cAMP-bound SpSLC9C1 in nanodiscs, dataset 5</name>
        <directory>/data/raw_220910_spSLC9C1-2N2-cAMP-FF8-bVK25s1_ngc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>2585</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.022</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.022</pixelHeight>
        </dimensions>
        <details>Unaligned movie frames (60 frames per movie), collected at 200 kV in counting mode. Total electron dose is 49.95 electrons/Å^2. Data were recorded with SerialEM using 3 x 3 multi-shot pattern. Movies are not gain-corrected, gain reference is provided.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned movie frames of cAMP-bound SpSLC9C1 in nanodiscs, dataset 6</name>
        <directory>/data/raw_220911_spSLC9C1-2N2-cAMP-FF8-bVK25s1_ngc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>1528</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.022</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.022</pixelHeight>
        </dimensions>
        <details>Unaligned movie frames (60 frames per movie), collected at 200 kV in counting mode. Total electron dose is 49.95 electrons/Å^2. Data were recorded with SerialEM using 3 x 3 multi-shot pattern. Movies are not gain-corrected, gain reference is provided.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned movie frames of cAMP-bound SpSLC9C1 in nanodiscs, dataset 7</name>
        <directory>/data/raw_220927_spSLC9C1-2N2-cAMP-FF8-bVK26s2_ngc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>1336</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.022</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.022</pixelHeight>
        </dimensions>
        <details>Unaligned movie frames (60 frames per movie), collected at 200 kV in counting mode. Total electron dose is 50.117 electrons/Å^2. Data were recorded with SerialEM using 3 x 3 multi-shot pattern. Movies are not gain-corrected, gain reference is provided.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned movie frames of cAMP-bound SpSLC9C1 in nanodiscs, dataset 8</name>
        <directory>/data/raw_221004_spSLC9C1-2N2-cAMP-FF8-bVK25s4_ngc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>2789</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.022</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.022</pixelHeight>
        </dimensions>
        <details>Unaligned movie frames (60 frames per movie), collected at 200 kV in counting mode. Total electron dose is 49.32 electrons/Å^2. Data were recorded with SerialEM using 3 x 3 multi-shot pattern. Movies are not gain-corrected, gain reference is provided.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned movie frames of cAMP-bound SpSLC9C1 in nanodiscs, dataset 9</name>
        <directory>/data/raw_221005_spSLC9C1-2N2-cAMP-FF8-bVK25s4_ngc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>2708</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.022</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.022</pixelHeight>
        </dimensions>
        <details>Unaligned movie frames (60 frames per movie), collected at 200 kV in counting mode. Total electron dose is 49.32 electrons/Å^2. Data were recorded with SerialEM using 3 x 3 multi-shot pattern. Movies are not gain-corrected, gain reference is provided.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned movie frames of cAMP-bound SpSLC9C1 in nanodiscs, dataset 10</name>
        <directory>/data/raw_221006_spSLC9C1-2N2-cAMP-FF8-bVK25s4_ngc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>1749</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.022</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.022</pixelHeight>
        </dimensions>
        <details>Unaligned movie frames (60 frames per movie), collected at 200 kV in counting mode. Total electron dose is 49.32 electrons/Å^2. Data were recorded with SerialEM using 3 x 3 multi-shot pattern. Movies are not gain-corrected, gain reference is provided.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned movie frames of cAMP-bound SpSLC9C1 in nanodiscs, dataset 11</name>
        <directory>/data/raw_221024_spSLC9C1-2N2-cAMP-FF8-bVK25s3_ngc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>6242</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.022</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.022</pixelHeight>
        </dimensions>
        <details>Unaligned movie frames (60 frames per movie), collected at 200 kV in counting mode. Total electron dose is 49.32 electrons/Å^2. Data were recorded with SerialEM using 3 x 3 multi-shot pattern. Movies are not gain-corrected, gain reference is provided.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned movie frames of cAMP-bound SpSLC9C1 in nanodiscs, dataset 12</name>
        <directory>/data/raw_220817_spSLC9C1-2N2-cAMP-bVK23s6_ngc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>138</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.022</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.022</pixelHeight>
        </dimensions>
        <details>Unaligned movie frames (60 frames per movie), collected at 200 kV in counting mode. Total electron dose is 53.599 electrons/Å^2. Data were recorded with SerialEM using 3 x 3 multi-shot pattern. Movies are not gain-corrected, gain reference is provided. This dataset was excluded from the original workflow.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
