REL 2023-07-11 2024-04-17 2024-04-17 0000-0001-7204-5863 Paul Victor Sauer QB3 Institute, University of California, Berkeley, CA, USA 742 Stanley Hall Berkeley CA United States 94720 0000-0002-9977-8482 Cheryl A Kerfeld MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA East Lansing MI United States 48824 0000-0001-9816-3681 Eva Nogales Molecular and Cell Biology Department, UC Berkeley Berkeley CA United States 94720 0000-0002-4394-0129 Benedetta Mennucci Dipartimento di Chimica e Chimica Industriale, University of Pisa Pisa Italy 56124 Sauer PV Kotecha A Koh AF 6.1 10.6019/EMPIAR-11644 EMDB molecule EMD-41463 EMD-41434 EMD-41435 EMD-41436 Sauer PV Cupellini L Sutter M Bondanza M Domínguez Martin MA Kirst H Bína D Koh AF Kotecha A Greber BJ Nogales E Polívka T Mennucci B Kerfeld CA Science advances Sci Adv 14 10 2024 English 10.1126/sciadv.adk7535 38578996 Unaligned, unsubtracted raw EER micrographs of OCP-PBS sample /data micrographs - multiframe EER EER 16285 1176 BIT 4096 0.727 4096 0.727

Streptavidin crystal lattice is present in micrographs, Dose 40 electrons per square Angstrom, Dose per frame = 40/1176 = 0.034 e/A2, Defocus -0.4 to -1.2 um, collected on Falcon 4, Krios 300 kV, Gain reference is included