REL
2023-09-05
2023-09-22
2023-09-22
Sheared F-actin raw data from Structures of the free and capped ends of the actin filament
0000-0003-3186-5229
Roberto
Dominguez
University of Pennsylvania
415 Curie Blvd.
Philadelphia
Pennsylvania
United States
19104
0000-0003-3186-5229
Roberto
Dominguez
University of Pennsylvania
415 Curie Blvd
Philadelphia
Pennsylvania
United States
19104
0000-0003-3186-5229
Roberto
Dominguez
University of Pennsylvania
415 Curie Blvd.
Philadelphia
Pennsylvania
United States
19104
Carman PJ
Barrie KR
Rebowski G
Dominguez R
National Institutes of Health (NIH)
GM073791
United States
7.3
10.6019/EMPIAR-11682
EMDB
molecule
EMD-28934
Carman PJ
Barrie KR
Rebowski G
Dominguez R
Structures of the free and capped ends of the actin filament
Science (New York, N.Y.)
Science
6651
380
1287
1292
2023
English
10.1126/science.adg6812
37228182
These are the raw movies used to generate the structure of the free barbed end of F-actin. Gain references are also provided.
Dataset 1 movies
/data/Beckman_10282022_sheared/movies
micrographs - multiframe
TIFF
TIFF
8760
42
UNSIGNED BYTE
11520
0.54
8184
0.54
Dataset 1 raw movies (*tiff format). Split into sub-directories with ~3000 movies each. The gain reference used for dataset 1 is located in directory /data/Beckman_10282022_sheared/gainref.
Pixel size = 0.54 A/pix (super-resolution, binned by 2 during motion correction)
Voltage = 300kV
Aberration = 2.7 mm
Dose = 44 e/A^2
Dataset 2 movies
/data/Beckman_04192023/movies
micrographs - multiframe
TIFF
TIFF
1502
40
UNSIGNED BYTE
11520
0.54
8184
0.54
Dataset 2 raw movies (*tiff format). The gain reference used for dataset 2 is located in directory /data/Beckman_04192023/gainref.
Pixel size = 0.54 A/pix (super-resolution, binned by 2 during motion correction)
Voltage = 300kV
Aberration = 2.7 mm
Dose = 43.7 e/A^2
Dataset 3 movies
/data/Beckman_04202023/movies
micrographs - multiframe
TIFF
TIFF
4372
40
UNSIGNED BYTE
11520
0.54
8184
0.54
Dataset 3 raw movies (*tiff format). Split into sub-directories with ~2200 movies each. The gain reference used for dataset 3 is located in directory /data/Beckman_04202023/gainref.
Pixel size = 0.54 A/pix (super-resolution, binned by 2 during motion correction)
Voltage = 300kV
Aberration = 2.7 mm
Dose = 43.7 e/A^2
Dataset 4 movies
/data/Beckman_04212023/movies
micrographs - multiframe
TIFF
TIFF
1947
40
UNSIGNED BYTE
11520
0.54
8184
0.54
Dataset 4 raw movies (*tiff format). The gain reference used for dataset 4 is located in directory /data/Beckman_04212023/gainref.
Pixel size = 0.54 A/pix (super-resolution, binned by 2 during motion correction)
Voltage = 300kV
Aberration = 2.7 mm
Dose = 43.7 e/A^2