REL 2023-07-20 2023-09-25 2023-09-25 Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex 0000-0002-0425-234X Rie Nygaard Department of Physiology and Cellular Biophysics at Columbia University 1150 Saint Nicolas Ave New York NY United States 10032 0000-0003-3293-2200 Filippo Mancia Columbia University 1150 Saint Nicolas Ave New York NY United States 10034 Nygaard R Graham CLB Belcher Dufrisne M Colburn JD Pepe J Hydorn MA Corradi S Brown CM Ashraf KU Vickery ON Briggs NS Deering JJ Kloss B Botta B Clarke OB Columbus L Dworkin J Stansfeld PJ Roper DI Mancia F 2.8 10.6019/EMPIAR-11685 EMDB molecule EMD-41299 EMD-41303 EMD-41304 Nygaard R Graham CLB Belcher Dufrisne M Colburn JD Pepe J Hydorn MA Corradi S Brown CM Ashraf KU Vickery ON Briggs NS Deering JJ Kloss B Botta B Clarke OB Columbus L Dworkin J Stansfeld PJ Roper DI Mancia F Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex Nature communications Nat Commun 1 14 2023 English 10.1038/s41467-023-40483-8 37620344
11,120 raw movie plus a gain reference micrograph. Pixel size 0.83A, accelerating voltage 300kV, spherical abberation 2.7mm, total exposure dose 58.5 e/A^2.
 Unaligned 50 frame micrographs of RodA-PBP2 in nanodisc /data micrographs - multiframe TIFF TIFF 11120 50 UNSIGNED BYTE 5760 0.83 4092 0.83
The micrographs are of 50 raw movie frames accumulating 58.5 electrons per squared Angstrom in a 2.5 second exposure. The data was collected on a 300 kV Krios and the spherical abberation is 2.7 mm.