REL 2023-09-27 2023-10-06 2023-10-06 CryoEM micrographs collected on a RAD51-ADP filament sample 0000-0002-9300-497X Luca Pellegrini University of Cambridge Sanger Building, 80 Tennis Ct Rd Cambridge Cambridgeshire United Kingdom CB21GA 0000-0002-9300-497X Luca Pellegrini University of Cambridge Sanger Building, 80 Tennis Ct Rd Cambridge Cambridgeshire United Kingdom CB21GA Appleby R Bollschweiler D Chirgadze DY Joudeh L Pellegrini L 1.5 10.6019/EMPIAR-11696 EMDB molecule EMD-16224 Appleby R Bollschweiler D Chirgadze DY Joudeh L Pellegrini L A metal ion-dependent mechanism of RAD51 nucleoprotein filament disassembly iScience iScience 5 26 2023 English 10.1016/j.isci.2023.106689 37216117
micrographs are averaged over 50 collection frames with a dose rate of 56.36 e/A2/s and exposure time of 1.34 seconds
 Micrographs of wildtype RAD51 filaments bound with ADP in the presence of dsDNA incl. gain reference /data micrographs - multiframe TIFF TIFF 9214 50 UNSIGNED BYTE 5760 variable 4092 variable
Data were collected using a Titan Krios G3 microscope fitted with a K3 detector operating at 300 keV. Micrographs were binned 2x in EPU prior to export giving a pixel size of 0.652A. Gain reference file for 2x binned data: data/20220711-130kx-K3-18210048_Gain_Ref.x1.m1-bin2.mrc
data/FoilHole_*_Data_*_fractions.tiff