<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11709" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2023-10-04</depositionDate>
            <releaseDate>2025-07-16</releaseDate>
            <updateDate>2025-07-16</updateDate>
        </keyDates>
        <title>A tomogram of a concave region on the nucleus of a neutrophil</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-7014-3635</authorORCID>
            <firstName>Zhixun</firstName>
            <lastName>Li</lastName>
            <organization type="academic">Peking University</organization>
            <townOrCity>Beijing</townOrCity>
            <country>China</country>
            <postOrZipCode>100000</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-3520-5439</authorORCID>
            <firstName>Qiang</firstName>
            <lastName>Guo</lastName>
            <organization type="academic">Peking University</organization>
            <townOrCity>Beijing</townOrCity>
            <country>China</country>
            <postOrZipCode>100000</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author>Liu JQ</author>
            <author authorORCID="0000-0002-7014-3635">Li ZX</author>
            <author authorORCID="0000-0003-3520-5439">Guo Q</author>
            <author>Du WJ</author>
            <author>Yang J</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Natural Science Foundation of China (NSFC)</fundingBody>
                <code>#32371191</code>
                <country>China</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="GB">35.7</datasetSize>
        <entryDOI>10.6019/EMPIAR-11709</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>cell</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-35979</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0009-0008-8857-8398" order="1">Liu J</author>
                    <author authorORCID="0000-0002-7014-3635" order="2">Li Z</author>
                    <author authorORCID="0000-0003-3931-7905" order="3">Li M</author>
                    <author authorORCID="0009-0009-0516-7946" order="4">Du W</author>
                    <author authorORCID="0000-0001-8154-8809" order="5">Baumeister W</author>
                    <author order="6">Yang J</author>
                    <author authorORCID="0000-0003-3520-5439" order="7">Guo Q</author>
                    <title>Vimentin regulates nuclear segmentation in neutrophils</title>
                    <journal>Proceedings of the National Academy of Sciences of the United States of America</journal>
                    <journalAbbreviation>Proc Natl Acad Sci U S A</journalAbbreviation>
                    <country>China</country>
                    <issue>48</issue>
                    <volume>120</volume>
                    <year>2023</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1073/pnas.2307389120</externalReferences>
                    <externalReferences type="pubmed">37983515</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned mutiframe micrographs of the concave nuclear region in a neutrophil</name>
        <directory>/data/Neutro_087</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>57</numImagesOrTiltSeries>
        <framesPerImage>10</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>1.66</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>1.66</pixelHeight>
        </dimensions>
        <details>Data was collected in super resolution mode. Frames before motion correction are in the folder: "frames". Segmentation is in the folder "Segmentation" (can be opened by ChimeraX). Coordinates for ribosomes and fibers are deposed in "ribo_coord" and "fiber_coord".</details>
        <segmentationList>
            <segmentation segmentationId="291">
                <file>data/Neutro_087/Segmentation</file>
                <description>The concave nuclear region in a neutrophil.</description>
            </segmentation>
        </segmentationList>
        <micrographsFilePattern>/data/Neutro_087/Segmentation/Neutro_tomo087/tomo_087_dose-filt_deconv.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/Neutro_087/ribo_coord/run_data.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/Neutro_087/ribo_coord</pickedParticlesDirectory>
    </imageSet>
</entry>
