REL 2023-10-11 2024-08-23 2024-08-23 Cyo-EM structure of wildtype non-gastric proton pump in the presence of Na+, AlF and ADP 0000-0003-2681-5921 Kazuhiro Abe Cellular and Structural Physiology Institute, Nagoya University 1, Furo-cho, Chikusa-ku Nagoya Aichi Japan 464-8601 0000-0003-2681-5921 Kazuhiro Abe Cellular and Structural Physiology Institute, Nagoya University 1, Furo-cho, Chikusa-ku Nagoya Aichi Japan 464-8601 Abe K Artigas P 1.7 10.6019/EMPIAR-11722 EMDB molecule EMD-35488 EMD-35489 EMD-35491 Abe K Nishizawa T Artigas P An unusual conformation from Na + -sensitive non-gastric proton pump mutants reveals molecular mechanisms of cooperative Na + -binding. Biochimica Biophysica Acta Molecular Cell Research Biochim Biophys Acta Mol Cell Res 1870 2023 English 10.1016/j.bbamcr.2023.119543 37482134
non-gastric proton pump WT E2-Pi state in the presence of Na+ AlF and ADP.
 K3 movies for non gastric proton pump WT with Na, AlF and ADP /data/8IJL/ micrographs - multiframe TIFF TIFF 1723 64 2 64 UNSIGNED BYTE 5760 0.83 4092 0.83
- Please use 'movie_gain_ref_estimate.mrc' as a gain reference without rotation or flipping in RELION. - The gain reference (movie_gain_ref_estimate.mrc) was generated with relion_estimate_gain by averaging many frames. - The flux is 1.06 e/A2/frame. - Cs is 2.7 mm. - Four digits in the file name represent image shift directions. - Movies were recorded with 3x3 image shift but grouping was not applied during processing.
 K3 movies for non gastric proton pump K794A mutant in NaE1AMPPCP state /data/8IJM/ micrographs - multiframe TIFF TIFF 3204 48 2 48 UNSIGNED BYTE 5760 0.83 4092 0.83
- Please use 'CountRef_abe1-3_11-001_0000_Sep09_09.26.25.mrc' as a gain reference without rotation or flipping in RELION. - The flux is 1.06 e/A2/frame. - Cs is 2.7 mm. - Four digits in the file name represent image shift directions. - Movies were recorded with 3x3 image shift but grouping was not applied during processing.
 K3 movies for non gastric proton pump K794S mutant in NaE1AMPPCP state /data/EMD-35491 micrographs - multiframe TIFF TIFF 2187 48 2 48 UNSIGNED BYTE 5760 0.83 4092 0.83
- Please use 'CountRef_abe2-1_22-001_0000_Sep10_01.28.37.mrc' as a gain reference without rotation or flipping in RELION. - The flux is 1.06 e/A2/frame. - Cs is 2.7 mm. - Four digits in the file name represent image shift directions. - Movies were recorded with 3x3 image shift but grouping was not applied during processing.