REL
2023-11-28
2024-04-02
2024-04-02
Cryo-EM of SARS-CoV-2 5' proximal stem-loops 5-6
0000-0001-7497-0972
Rhiju
Das
Department of Biochemistry, Stanford University; Howard Hughes Medical Institute
279 Campus Dr W
Stanford
CA
United States
94305
0000-0002-8910-3078
Wah
Chiu
Department of Bioengineering, James H. Clark Center, Stanford University; CryoEM and Bioimaging Division, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory
318 CAMPUS DR
Stanford
CA
United States
94305
Kretsch RC
Xu L
Zheludev IN
Zhou X
Huang R
Nye G
Li S
Zhang K
Chiu W
Das R
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R35GM122579
United States
Howard Hughes Medical Institute (HHMI)
N/A
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
U24GM129541
United States
National Science Foundation (NSF, United States)
GRFPDGE1656518
United States
2.8
10.6019/EMPIAR-11813
EMDB
molecule
EMD-42821
Kretsch RC
Xu L
Zheludev IN
Zhou X
Huang R
Nye G
Li S
Zhang K
Chiu W
Das R
Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses
Proceedings of the National Academy of Sciences of the United States of America
Proc Natl Acad Sci U S A
10
121
2024
English
10.1073/pnas.2320493121
38427602
Kretsch RC
Xu L
Zheludev IN
Zhou X
Huang R
Nye G
Li S
Zhang K
Chiu W
Das R
Tertiary folds of the SL5 RNA from the 5′ proximal region of SARS-CoV-2 and related coronaviruses
bioRxiv
10.1101/2023.11.22.567964
Unaligned multi-frame micrographs of SARS-CoV-2 5' stem-loops 5-6 RNA.
/data/SARS-CoV-2_SL5-6/exposures
micrographs - multiframe
TIFF
TIFF
1763
40
SIGNED 16 BIT INTEGER
4096
1.0
4096
1.0
These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector. Over the collection, the sample was exposed evenly to 50 electrons per angstrom squared with a total exposure time of 8.50 seconds. Desired defocuses ranged from -1.2 to -2.5 nm. The exposures are gain corrected.
data/SARS-CoV-2_SL5-6/exposures/GridSquare_*/*_Fractions.mrc
Extracted particles and orientations for final reconstruction of SARS-CoV-2 5' stem-loops 5-6 RNA.
/data/SARS-CoV-2_SL5-6/particles
picked particles - single frame - processed
MRC
MRC
99785
1
32 BIT FLOAT
420
1.0
420
1.0
Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/SARS-CoV-2_SL5-6/particles/P3_J1399_particles_exported.cs.
data/SARS-CoV-2_SL5-6/particles/data/*mrc
data/SARS-CoV-2_SL5-6/particles/data