REL
2023-11-28
2025-02-04
2025-02-04
Cryo-EM of Tetrahymena ribozyme
0000-0001-7497-0972
Rhiju
Das
Department of Biochemistry, Stanford University; Howard Hughes Medical Institute
279 Campus Dr W
Stanford
CA
United States
94305
0000-0002-8910-3078
Wah
Chiu
Department of Bioengineering, James H. Clark Center, Stanford University; CryoEM and Bioimaging Division, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory
318 CAMPUS DR
Stanford
CA
United States
94305
Kretsch RC
Li S
Pintilie G
Palo M
Case D
Zhang K
Das R
Chiu W
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R35GM122579
United States
Howard Hughes Medical Institute (HHMI)
N/A
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
U24GM129541
United States
3.7
10.6019/EMPIAR-11844
EMDB
molecule
EMD-42498
EMD-42499
Kretsch RC
Li S
Pintilie G
Palo MZ
Case DA
Das R
Zhang K
Chiu W
Complex Water Networks Visualized through 2.2-2.3 Angstrom Cryogenic Electron Microscopy of RNA
bioRxiv
2025
10.1101/2025.01.23.634578
Unaligned multi-frame micrographs of tetrahymena ribozyme.
/data/ThR/exposures
micrographs - multiframe
TIFF
TIFF
18366
30
UNSIGNED BYTE
5760
0.82
4092
0.82
These micrographs were collected with a Titan Krios G3i, 300kV and a K3 detector. Over the collection, the sample was exposed evenly to 57.25 electrons per angstrom squared with a total exposure time of 2.5 seconds. Desired defocuses ranged from -0.5 to -2 nm. The exposures are not gain corrected, the gain reference can be found at data/ThR/exposures/K3-18210041 Gain Ref. x1.m1.mrc.
data/ThR/exposures/GridSquare_*/*_fractions.tiff
Extracted particles and orientations for final reconstruction of tetrahymena ribozyme.
/data/ThR/particles
picked particles - single frame - processed
MRC
MRC
1181000
1
32 BIT FLOAT
256
0.82
256
0.82
Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/ThR/particles/2.2A_cryosparc_P5_J1222_004_particles.cs and data/ThR/particles/2.3A_cryosparc_P5_J1224_004_particles.cs
data/ThR/particles/data_*/*mrcs
data/ThR/particles/data_*