REL 2024-04-09 2025-07-10 2025-07-10 0000-0001-6861-4840 Yukihiko Sugita Division of Ultrastructural Virology, Institute for Life and Medical Sciences Division of Ultrastructural Virology, Institute for Life and Medical Sciences Sakyo-ku Kyoto Japan 6068397 0000-0002-2001-9388 Toyoyuki Ose Hokkaido University N10W8 Sapporo Hokkaido Japan 060-0810 Minami M Sugiyama A Hirose M Sugita Y Ose T 1.9 10.6019/EMPIAR-12034 EMDB molecule EMD-39679 Minami M Ugajin K Inaba-Inoue S Yabuno N Takekawa Y Xiaomei S Takei S Sasaki M Nomai T Jiang X Kita S Maenaka K Hirose M Yao M Gooley PR Moseley GW Sugita Y Ose T Science signaling Sci Signal 878 18 2025 English 10.1126/scisignal.ads2210 40100957 6993 K3 movies in TIFF /data/221010_slot4_frames micrographs - multiframe TIFF TIFF 6993 54 UNSIGNED BYTE 5760 0.87 4092 0.87

- Please use relion_gain.mrc as a gain reference without flipping or rotation. - The dataset was collected with 3x3 image shifts. The shift direction is indicated in the file name (e.g. "X+0Y+0"). Data processing was done with a single optics group. - The scope has a Cs corrector. The Cs after correction is 0.032 mm. - The pixel size in the image header (0.87 Å/px) is not accurate. The right value is 0.88 Å/px. - The flux is 0.93 e/Å^2/frame.