REL 2024-02-16 2025-05-04 2025-05-04 Structural basis for translational control by the human 48S initiation complex 0000-0002-4609-4052 Niels Fischer Max Planck Institute for Multidisciplinary Sciences Am Fassberg 11 Goettingen Niedersachsen Germany 37077 0000-0002-4609-4052 Niels Fischer Max Planck Institute for Multidisciplinary Sciences Am Fassberg 11 Goettingen Niedersachsen Germany 37077 Petrychenko V Yi SH Liedtke D Peng BZ Rodnina MV Fischer N 6.9 10.6019/EMPIAR-12094 EMDB molecule EMD-17696 EMD-17697 EMD-17698 EMD-17699 EMD-17700 EMD-17701 EMD-19128 Petrychenko V Yi SH Liedtke D Peng BZ Rodnina MV Fischer N Structural basis for translational control by the human 48S initiation complex Nature structural & molecular biology Nat Struct Mol Biol 1 32 62 72 2024 English 10.1038/s41594-024-01378-4 39289545 Motion-corrected, dose-weighted micrographs /data/corrected_micrographs micrographs - single frame MRC MRC 93510 1 32 BIT FLOAT 4096 1.16 4096 1.16
Micrographs were obtained from movie-stacks of 20 frames à 2-2.5 e/A^2 by global motion-correction and dose-weighting using Relion 4.0, CTF parameters were estimated with GCTF 1.06 and particles were selected by GAUTOMATCH 0.56. - Micrographs star-file (Micrographs with FinalResolution better 4.5A): data/corrected_micrographs.star - Particle coordinates: data/corrected_micrographs/*_automatch.star
data/corrected_micrographs/FoilHole_*_Data_*_Fractions.mrc Shiny particles, obtained by Bayesian polishing in Relion 4.0 /data/particles picked particles - single frame - processed MRCS MRCS 1308019 1 32 BIT FLOAT 360 1.16 360 1.16
Star-files for individual particle populations are located in data/particles (see below), masks used for sorting and final refinements in data/particles/masks360. Star-file for all shiny particles: - data/shiny_particles360_all.star Star-files for shiny particles of major states - data/particles/shiny_particles360_48S-1.star - data/particles/shiny_particles360_48S-2.star - data/particles/shiny_particles360_48S-3.star - data/particles/shiny_particles360_48S-4.star - data/particles/shiny_particles360_48S-5.star Star-files for shiny particles of eIF2, tRNA and eIFB5 substates: - data/particles/shiny_particles360_48S-2-1.star - data/particles/shiny_particles360_48S-2-2.star - data/particles/shiny_particles360_48S-2-3.star - data/particles/shiny_particles360_48S-3-1.star - data/particles/shiny_particles360_48S-3-2.star - data/particles/shiny_particles360_48S-3-3.star - data/particles/shiny_particles360_48S-4-1.star - data/particles/shiny_particles360_48S-4-2.star - data/particles/shiny_particles360_48S-5-1.star - data/particles/shiny_particles360_48S-5-2.star - data/particles/shiny_particles360_48S-5-3.star Star-files for shiny particles of eIF3bi substates: - data/particles/shiny_particles360_48S-1_eIF3bi_majorstate.star - data/particles/shiny_particles360_closed48S_eIF3bi_majorstate.star - data/particles/shiny_particles360_closed48S_eIF3bi_minorstate1.star - data/particles/shiny_particles360_closed48S_eIF3bi_minorstate2.star
data/corrected_micrographs/FoilHole_*_Data_*_Fractions.mrc data/particles/shiny_particles360/job*/*/FoilHole_*_Data_*_Fractions_shiny.mrcs data/particles/shiny_particles360/job*/* Signal-subtracted particles - eIF3core in open 48S /data/subtracted_particles/subtracted_particles240_eIF3core_open48S picked particles - single frame - processed MRCS MRCS 92749 1 32 BIT FLOAT 240 1.16 240 1.16
Star-files with subtracted particles used for local refinement of eIF3core in open 48S (48S-1): - data/subtracted_particles/subtracted_particles240_eIF3core_open48S.star Masks used for refinements are located in data/subtracted_particles/masks240.
data/corrected_micrographs/FoilHole_*_Data_*_Fractions.mrc data/subtracted_particles/subtracted_particles240_eIF3core_open48S/subtracted_rank*_opticsgroup*.mrcs data/subtracted_particles/subtracted_particles240_eIF3core_open48S Signal-subtracted particles - eIF3core in closed 48S /data/subtracted_particles/subtracted_particles240_eIF3core_closed48S picked particles - single frame - processed MRCS MRCS 356632 1 32 BIT FLOAT 240 1.16 240 1.16
Star-files with subtracted particles used for local refinement of eIF3core in closed 48S (48S-2 to 5): - data/subtracted_particles/subtracted_particles240_eIF3core_closed48S.star Masks used for refinements are located in data/subtracted_particles/masks240.
data/corrected_micrographs/FoilHole_*_Data_*_Fractions.mrc data/subtracted_particles/subtracted_particles240_eIF3core_closed48S/subtracted_rank*_opticsgroup*.mrcs data/subtracted_particles/subtracted_particles240_eIF3core_closed48S