TIR Name of tilt-series and tomogram after renaming Lamella_ID number identifieer of the lamella the tilt-series was acquired on excluded Tomograms excluded from further processing (see materials and methods for criteria) excluded_M Excluded during M processing, due to software error Thickness (nm) Local thickness value of each tomogram, obtained from minimal distance between surface annotations GIS (um) Distance of centre of position where tilt-series were acquired to the GIS layer distance_backside (um) Distance of the centre of position where tilt-series were acquired to the amorphous area on the backside of the lamellae cryolo_ribo number of ribosomes identified by cryolo model (trained and applied on Isonet filtered tomograms) class_ribo number of ribosomes identified after classification post_M Number of ribosomes retained post TIR Lamella_ID excluded excluded_M Thickness (nm) GIS (um) distance_backside (um) cryolo_ribo class_ribo Comment TIR239 1 241 899 313 TIR240 3 212 6.4 8.5 716 400 TIR241 3 1 8.2 8 128 92 TIR242 3 243 9.3 8.2 548 349 TIR243 3 203 10.6 3.9 628 273 TIR244 3 1 9.5 3.4 106 72 TIR245 3 212 13.2 3.5 836 374 TIR246 3 186 12.3 1.5 1126 683 TIR247 3 189 5.3 12 1246 805 TIR248 3 140 2.4 7.9 713 548 TIR249 3 116 1.1 8.3 160 84 TIR250 3 122 0.6 10.2 710 367 TIR251 5 172 4.7 12.8 1183 817 TIR252 5 193 5.6 12.9 1129 427 TIR253 5 158 1.8 11.3 858 379 TIR254 5 161 3.1 12 714 375 TIR255 5 1 1.6 17 7 2 TIR256 5 140 1.1 13.6 940 558 TIR257 5 176 6.2 11.2 1065 482 TIR258 5 208 7.4 9.9 940 382 TIR259 5 209 8.4 10.2 1281 608 TIR260 5 217 9.5 9.5 835 383 TIR261 5 183 4.8 9.4 1234 658 TIR262 5 181 4.4 8.7 1002 432 TIR263 5 193 6.7 8 1255 722 TIR264 5 202 10.1 8.5 1278 704 TIR265 5 153 2.2 9.1 919 463 TIR266 5 1 11.6 6.9 1160 110 TIR267 5 1 12.2 6.8 915 111 TIR268 5 1 10.6 6.9 1033 137 TIR269 5 182 8.7 4.3 1586 1001 TIR270 5 167 6.7 4.6 1013 727 TIR271 5 180 6.5 5.2 759 438 TIR272 5 1 12.5 4.8 450 178 TIR273 5 1 13.3 4.6 1223 160 TIR274 5 1 10.8 2.8 1288 155 TIR275 5 1 8.6 3.8 56 1 TIR276 5 1 13.5 2.8 1056 187 TIR277 5 1 14.5 3.2 1089 294 TIR278 5 1 10.9 1.7 948 120 TIR279 5 1 6 3.1 984 148 TIR280 5 1 11.8 1.3 955 254 TIR281 5 1 13.3 1.4 271 22 TIR282 5 175 10.3 0.4 903 658 TIR283 5 185 8.9 0.6 907 198 TIR284 5 174 11.8 0.1 768 40 TIR285 5 160 4.2 7.2 891 548 TIR286 6 1 10.3 0.6 822 382 TIR287 6 185 9.8 0.9 675 463 TIR288 6 1 12.2 0.9 153 7 TIR289 6 247 15.1 1.6 1244 596 TIR290 6 1 13.5 2.4 125 1 TIR291 6 272 16.5 0.7 768 273 TIR292 6 268 14.9 2.4 1165 306 TIR293 6 216 10.3 2.9 594 347 TIR294 6 200 9.4 2.5 413 36 TIR295 6 242 13.1 3.6 1559 689 TIR296 6 256 13.8 3.7 988 249 TIR297 6 259 11.5 5.7 1773 505 TIR298 6 210 7.2 3.6 456 216 TIR299 6 1 209 7 3.4 782 145 TIR300 6 246 11 7.1 815 10 TIR301 7 209 12.8 1.4 1127 333 TIR302 7 222 14.3 0.9 988 379 TIR303 7 238 15.2 0.6 1114 529 TIR304 7 1 462 212 TIR305 7 219 13.6 1.9 1117 481 TIR306 7 185 11.5 3.7 771 418 TIR307 7 211 12.5 2.6 860 670 TIR308 7 242 13 3.1 1015 561 TIR309 7 184 8.1 5.2 1199 570 TIR310 7 167 9 4.6 711 364 TIR311 7 189 9.3 4.7 1097 629 TIR312 7 201 10.2 1.7 1418 969 TIR313 7 1 209 13 0.4 736 379 TIR314 7 1 180 10.5 2.7 858 188 TIR315 7 180 3.8 11.7 1243 958 TIR316 7 218 5.2 10.8 1114 599 TIR317 7 1 69 0 TIR318 7 1 570 328 TIR319 7 191 1335 1019 TIR320 7 207 8.7 6.8 1261 848 TIR321 7 1 269 1333 476 TIR322 8 223 13.1 2.2 1323 744 TIR323 8 1 15.4 2.5 0 0 TIR324 8 229 11.8 3.9 1487 693 TIR325 8 270 10.7 4.2 852 62 TIR326 8 223 15 3.2 843 528 TIR327 8 212 8.2 6.2 581 50 TIR328 8 1 211 10.6 5.6 1084 562 TIR329 8 184 7 6.4 725 412 TIR330 8 227 8.7 7.8 1247 745 TIR331 8 246 9.9 7.6 1453 884 TIR332 8 215 6.1 8.5 1156 691 TIR333 8 172 1 14.6 1026 731 TIR334 8 150 1.5 7.4 532 348 TIR335 8 169 3.5 7.74 794 422 TIR336 8 155 2 10.3 1203 709 TIR337 8 1 164 0.6 12 469 250 TIR338 13 195 12.3 1.7 654 349 TIR339 13 1 13.7 -0.6 5 0 TIR340 13 209 10.6 4.4 875 324 TIR341 13 211 11 3.3 807 205 TIR342 13 272 11.4 4 1089 152 TIR343 13 201 8.8 5.6 699 327 TIR344 13 191 6.5 7.4 1064 490 TIR345 13 171 4.4 9.9 1285 952 TIR346 13 140 1.2 12.6 853 606 TIR347 11 1 199 13.4 0.7 1007 650 TIR348 11 193 14.5 0.5 1073 562 TIR349 11 193 13.2 1.4 1353 416 TIR350 11 1 11.8 1.8 127 10 TIR351 11 197 11.7 2.9 978 595 TIR352 11 193 10.4 3.3 959 536 TIR353 11 188 10.2 3.7 1024 474 TIR354 11 131 8.4 5.8 564 361 TIR355 11 1 50 3 TIR356 11 1 370 173 TIR357 11 1 70 8 TIR358 11 197 9 6.3 1236 645 TIR359 11 133 9.5 5.8 910 523 TIR360 11 121 11.2 4.1 368 223 TIR361 11 182 13.5 1.4 1147 763 TIR362 11 165 14 0.9 652 304 TIR363 16 266 11.5 1.3 1399 439 TIR364 16 1 775 8 TIR365 16 1 13 2 TIR366 16 1 1236 122 TIR367 16 1 951 52 TIR368 16 1 1199 20 TIR369 16 1 1061 50 TIR370 16 1 941 30 TIR371 16 1 624 25 TIR372 16 1 143 0 TIR373 16 1 1184 43 TIR374 16 1 285 3 TIR375 16 1 1323 42 TIR376 16 1 725 24 TIR377 16 1 29 2 TIR378 16 1 24 1 TIR379 16 1 166 5 TIR380 16 1 920 305 TIR381 16 1 889 218 TIR382 16 1 305 36 TIR383 17 186 9.3 1 1068 684 TIR384 17 1 10.9 1.1 345 61 TIR385 17 1 9.8 1 1278 905 TIR386 17 172 6.5 2.6 871 563 TIR387 17 187 8.5 2 782 377 TIR388 17 179 9.5 2.1 810 135 TIR389 17 154 4.7 3.8 1211 920 TIR390 17 189 8.1 3.6 1161 733 TIR391 17 138 3.4 6.1 1093 772 TIR392 17 156 3.2 7.3 785 540 TIR393 17 186 6.3 6.7 1001 564 TIR394 17 232 15.2 0.8 1403 469 TIR395 17 218 15.9 0.8 938 453 TIR396 17 193 13.2 1 1097 579 TIR397 17 219 13.5 2.6 1492 929 TIR398 17 234 16.1 1.7 1038 620 TIR399 17 183 11.9 2.3 952 511 TIR400 17 202 11.1 2.5 1036 467 TIR401 17 191 9.9 3.7 1207 776 TIR402 17 215 10.7 4.5 1058 612 TIR403 17 192 9.5 4.8 1156 763 TIR404 17 158 3.1 9 990 617 TIR405 17 175 4.9 8.3 576 308 TIR406 17 173 3.3 8.3 1087 645 TIR407 17 165 1.8 9 1196 901 TIR408 17 215 11.2 6.7 1269 812 TIR409 17 1 212 7.9 7.6 1016 446 TIR410 17 220 7 9.1 1522 876 TIR411 17 214 9.4 7.9 938 293 TIR412 17 1 8.4 7.9 895 342 TIR413 17 200 6.9 10.2 1338 956 TIR414 17 194 6.8 10.5 1361 898 TIR415 17 179 4.8 12.8 1276 928 TIR416 10 134 1.9 7.2 924 650 TIR417 10 156 3.1 6.9 824 571 TIR418 10 151 5.2 7.2 356 51 TIR419 10 1 7 1 TIR420 10 1 126 1 TIR421 10 1 22 1 TIR422 10 1 0 0 TIR423 10 202 11.7 4.7 1424 853 TIR424 10 1 31 0 TIR425 10 1 95 3 TIR426 10 161 3.9 10.5 637 360 TIR427 10 1 257 171 TIR428 9 225 7.6 0.9 494 318 TIR429 9 230 7.5 0.4 1063 701 TIR430 9 259 9.1 0.9 1137 588 TIR431 9 232 6.7 1.7 1295 373 TIR432 9 261 7.8 1.9 1489 431 TIR433 9 267 7.2 2.8 1191 684 TIR434 9 249 6 3.7 623 50 TIR435 9 225 3.7 4.3 822 493 TIR436 9 1 133 0 TIR437 9 236 3.2 6.2 1129 590 TIR438 9 205 2 5.6 1255 793 TIR439 9 247 3.9 6.3 622 299 TIR440 9 1 262 3.9 7.8 939 530 TIR441 9 242 1023 491 TIR442 9 261 3.7 7.1 798 438 TIR443 9 1 14.8 0.7 233 28 TIR444 9 1 351 21 TIR445 9 1 45 2 TIR446 9 1 669 66 TIR447 9 1 402 19 TIR448 9 1 906 286 TIR449 19 1 920 73 TIR450 19 1 996 72 TIR451 19 1 398 8 TIR452 19 1 427 10 TIR453 19 1 843 28 TIR454 19 1 796 33 TIR455 19 1 551 12 TIR456 19 1 523 30 TIR457 19 1 1149 155 TIR458 19 1 728 13 TIR459 19 1 138 1 TIR460 19 1 1 0 TIR461 19 1 759 13 TIR462 19 1 500 14 TIR463 19 1 896 18 TIR464 19 1 522 6 TIR465 19 1 262 6 TIR466 19 1 327 10 TIR467 18 1 238 11 TIR468 18 234 12.5 0.5 515 100 TIR469 18 1 161 31 TIR470 18 248 12.2 1.4 1309 482 TIR471 18 1 262 12.8 1.8 838 183 TIR472 18 227 8.4 2.6 1363 425 TIR473 18 1 47 0 TIR474 18 211 4.2 2.7 697 404 TIR475 18 208 3.7 4.1 633 399 TIR476 18 205 5.3 4.8 674 112 TIR477 18 206 3.1 9.3 989 66 TIR478 20 1 194 90 TIR479 20 1 271 81 TIR480 20 1 78 13 TIR481 20 1 76 0 TIR482 20 1 5 0 TIR483 20 1 37 0 TIR484 20 1 2 0 TIR485 20 1 46 0 TIR486 20 1 1 0 TIR487 20 1 30 0 TIR488 20 1 46 0 TIR489 20 1 469 1 TIR490 20 229 12.5 4.8 1158 372 TIR491 20 1 460 12 TIR492 20 229 11.8 5.6 570 168 TIR493 20 1 61 1 TIR494 20 197 10.2 7.4 949 520 TIR495 20 1 46 0 TIR496 20 218 10.1 7.3 537 184 TIR497 20 1 197 10.6 6 1047 516 TIR498 20 219 8.5 9.2 1358 472 TIR499 20 1 397 23 TIR500 20 201 7.8 9.5 465 198 TIR501 20 240 8.3 9.7 665 199 TIR502 20 193 8.5 8.3 931 501 TIR503 20 1 200 29 TIR504 20 202 5.7 11.9 969 405 TIR505 20 248 6.4 11.8 1171 355 TIR506 20 188 6.1 10.9 947 681 TIR507 20 242 565 276 TIR508 20 180 3.2 14.3 944 588 TIR509 20 170 2.4 15 429 191 TIR510 20 1 10.1 5.5 178 14 TIR511 20 211 7.1 5.5 846 432 TIR512 20 227 5.5 9.2 597 300 TIR513 20 188 7.1 8.4 689 409 TIR514 20 207 5.9 9.4 721 317 TIR515 20 1 215 5.3 8.2 1011 444 TIR516 20 200 4.5 10.6 925 444 TIR517 20 177 6.5 9.6 745 388 TIR518 20 1 177 5.4 10.6 904 527 TIR519 20 191 4.4 10.4 650 290 TIR520 20 197 1.5 10.9 549 302 TIR521 8 1 574 16 Excluded test lamella TIR522 8 1 326 6 Excluded test lamella TIR523 8 1 46 3 Excluded test lamella TIR524 8 1 699 48 Excluded test lamella TIR525 8 1 828 49 Excluded test lamella TIR526 6 1 952 527 TIR527 6 1 904 399 TIR528 6 210 7.3 7 889 484 TIR529 6 1 271 88