REL
2024-10-29
2025-02-03
2025-02-03
Single particle reconstruction of human apo TRPM8 channel in a closed state
0000-0001-5582-2851
Mariusz
Czarnocki-Cieciura
International Institute of Molecular and Cell Biology in Warsaw
4 Ks. Trojdena Street
Warsaw
Poland
02-109
0000-0001-8632-0977
Marcin
Nowotny
International Institute of Molecular and Cell Biology in Warsaw
4 Ks. Trojdena Street
Warsaw
Poland
02-109
Palchevskyi S
Czarnocki-Cieciura M
Vistoli G
Gervasoni S
Nowak E
Beccari AR
Nowotny M
Talarico C
Other government
1/SOL/2021/2
Poland
Other government
F/090033/01-03-04/X36
Italy
3.4
10.6019/EMPIAR-12443
EMDB
molecule
EMD-15981
EMD-15982
EMD-15983
Palchevskyi S
Czarnocki-Cieciura M
Vistoli G
Gervasoni S
Nowak E
Beccari AR
Nowotny M
Talarico C
Structure of human TRPM8 channel
Communications biology
Commun Biol
1
6
2023
English
10.1038/s42003-023-05425-6
37857704
Unaligned multi-frame movies of human apo TRPM8 channel in a closed state
/data/Movies
micrographs - multiframe
TIFF
TIFF
7918
40
UNSIGNED BYTE
5760
0.82
4092
0.82
Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU). Voltage: 300 kV; spherical aberration: 2.7; dose per frame: 1 e/A^2 (total dose: 41.42 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.7, -2.4, -2.1, -1.8, -1.5, -1.2, -0.9 µm. Objective aperture retracted, C2 aperture: 50 µm.
data/Movies/*.tiff
data/TRPM8_EMD-15982/EMD-15982_particlestack.star
data/TRPM8_EMD-15982
Aligned particles of human apo TRPM8 channel in a closed state (consensus map)
/data/TRPM8_EMD-15982
picked particles - single frame - processed
MRCS
MRCS
110176
1
32 BIT FLOAT
420
0.82
420
0.82
Aligned particles with corresponding Relion-3 STAR file for the consensus TRPM8 reconstruction. Coordinates correspond to micrographs binned 2x during Motion correction.
data/Movies/*.tiff
data/TRPM8_EMD-15982/EMD-15982_particlestack.star
data/TRPM8_EMD-15982
Aligned particles of human apo TRPM8 channel in a closed state (focused pre-MHR + MHR1/2 map)
/data/TRPM8_EMD-15983
picked particles - single frame - processed
MRCS
MRCS
212851
1
32 BIT FLOAT
192
0.82
192
0.82
Aligned particles with corresponding Relion-3 STAR file for the focused TRPM8 reconstruction (pre-MHR + MHR1/2 map). Particles were signal-subtracted; coordinates correspond to micrographs binned 2x during Motion correction.
data/Movies/*.tiff
data/TRPM8_EMD-15983/EMD-15983_particlestack.star
data/TRPM8_EMD-15983