<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12458" schemaVersion="0.65" public="true">
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        <keyDates>
            <depositionDate>2024-10-31</depositionDate>
            <releaseDate>2025-08-01</releaseDate>
            <updateDate>2025-08-01</updateDate>
        </keyDates>
        <title>Dataset 2: Complex 2,3 and 4:  30S ribosome with IF1/2/3, tRNA, and GE81112 in complex preparation reaction</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-7807-7049</authorORCID>
            <firstName>Sean</firstName>
            <middleName>R</middleName>
            <lastName>Connell</lastName>
            <organization type="academic">Biobizkaia</organization>
            <townOrCity>barakaldo</townOrCity>
            <country>Spain</country>
            <postOrZipCode>48903</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-7807-7049</authorORCID>
            <firstName>Sean</firstName>
            <middleName>R</middleName>
            <lastName>Connell</lastName>
            <organization type="academic">Biobizkaia</organization>
            <townOrCity>barakaldo</townOrCity>
            <country>Spain</country>
            <postOrZipCode>48903</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author>Schedlbauer A</author>
            <author>Han X</author>
            <author>van Bakel W</author>
            <author>Kaminishi T</author>
            <author>Ochoa-Lizarralde B</author>
            <author>Iturrioz I</author>
            <author>Çapuni R</author>
            <author>Parry R</author>
            <author>Zegarra R</author>
            <author>Gil-Carton D</author>
            <author>López-Alonso JP</author>
            <author>Sanz KB</author>
            <author>Brandi L</author>
            <author>Gualerzi CO</author>
            <author>Fucini P</author>
            <author>Connell SR</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Ministry of Economy and Competitiveness (MINECO)</fundingBody>
                <code>PID2021-122705OB-I00</code>
                <country>Spain</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">3.6</datasetSize>
        <entryDOI>10.6019/EMPIAR-12458</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-51965</emdbEntry>
            <emdbEntry>EMD-51966</emdbEntry>
            <emdbEntry>EMD-51967</emdbEntry>
            <emdbEntry>EMD-51968</emdbEntry>
            <emdbEntry>EMD-51969</emdbEntry>
            <emdbEntry>EMD-51970</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="true">
                    <author order="1">Schedlbauer A</author>
                    <author order="2">Han X</author>
                    <author order="3">van Bakel W</author>
                    <author order="4">Kaminishi T</author>
                    <author order="5">Ochoa-Lizarralde B</author>
                    <author order="6">Iturrioz I</author>
                    <author order="7">Çapuni R</author>
                    <author order="8">Parry R</author>
                    <author order="9">Zegarra R</author>
                    <author order="10">Gil-Carton D</author>
                    <author order="11">López-Alonso JP</author>
                    <author order="12">Sanz KB</author>
                    <author order="13">Brandi L</author>
                    <author order="14">Gualerzi CO</author>
                    <author order="15">Fucini P</author>
                    <author order="16">Connell SR</author>
                    <title>A binding site for the antibiotic GE81112 in the ribosomal mRNA channel</title>
                    <journal>bioRxiv</journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                    <year>2024</year>
                    <externalReferences type="doi">10.1101/2024.09.26.614503</externalReferences>
                    <details>Dataset 2: Complex 2,3 and 4:  30S ribosome with IF1/2/3, tRNA, and GE81112 in complex preparation reaction 
6172 gain corrected movies. 1.113 Raw pixel size (A); 300 Accelerating voltage (kV); 2.7 Spherical aberration (mm);  44.08 Total exposure dose (e/A^2)</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>unaligned multiframe movies from dataset 2: 30S + IF1/2/3 +mRNA + tRNA GE81112</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>6172</numImagesOrTiltSeries>
        <framesPerImage>19</framesPerImage>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>1.113</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>1.113</pixelHeight>
        </dimensions>
        <details>unaligned multiframe movies from dataset 2: 30S + IF1/2/3 +mRNA + tRNA GE81112.  Total Exposure (e-/ Å 2)
44.08; Voltage (kV) 300</details>
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        <micrographsFilePattern>data/FoilHole_*_Fractions.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
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