<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12573" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2025-02-14</depositionDate>
            <releaseDate>2025-12-07</releaseDate>
            <updateDate>2025-12-07</updateDate>
        </keyDates>
        <title>Cryo electron micrographs of Arabidopsis thaliana H2A.W-H3.3-nucleosome with Arabidopsis native 147bp DNA 15.2.2</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-6786-9032</authorORCID>
            <firstName>Youchao</firstName>
            <lastName>Wang</lastName>
            <organization type="academic">State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University</organization>
            <townOrCity>Shanghai</townOrCity>
            <country>China</country>
            <postOrZipCode>200438</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-2075-4235</authorORCID>
            <firstName>Aiwu</firstName>
            <lastName>Dong</lastName>
            <organization type="academic">State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University</organization>
            <townOrCity>Shanghai</townOrCity>
            <country>China</country>
            <postOrZipCode>200438</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-6786-9032">Wang Y</author>
            <author authorORCID="0000-0002-2075-4235">Dong A</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Natural Science Foundation of China (NSFC)</fundingBody>
                <code>31930017</code>
                <country>China</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">1.5</datasetSize>
        <entryDOI>10.6019/EMPIAR-12573</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-62047</emdbEntry>
            <emdbEntry>EMD-62048</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="true">
                    <author authorORCID="0000-0001-6786-9032" order="1">Wang Y</author>
                    <title>unpublished</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Cryo electron micrographs of Arabidopsis thaliana H2A.W-H3.3-nucleosome with Arabidopsis native 147bp DNA 15.2.2</name>
        <directory>/data/Movies</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>1637</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>1.072</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>1.072</pixelHeight>
        </dimensions>
        <details>These micrographs doesn't need a gain file.</details>
        <segmentationList/>
        <micrographsFilePattern>data/Movies/*.tiff</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Particles for cryo electron micrographs of Arabidopsis thaliana H2A.W-H3.3-nucleosome with Arabidopsis native 147bp DNA 15.2.2</name>
        <directory>/data/cryosparc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRCS</headerFormat>
        <dataFormat>MRCS</dataFormat>
        <numImagesOrTiltSeries>1067</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>16 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>256</imageWidth>
            <pixelWidth>1.072</pixelWidth>
            <imageHeight>256</imageHeight>
            <pixelHeight>1.072</pixelHeight>
        </dimensions>
        <details>The folder cryosparc/J1 is "Imported particles" of CryoSPARC-v4.3.1, and the particles is imported from "Particle extraction" of relion-v3.1.3.
The folder cryosparc/J11 is "Homogeneous Refinement" of CryoSPARC-v4.3.1, and this "Homogeneous Refinement" which imposed C1 symmetry is at a resolution of 2.97 angstrom.
The folder cryosparc/J20 is "Homogeneous Refinement" of CryoSPARC-v4.3.1, and this "Homogeneous Refinement" which imposed C2 symmetry is at a resolution of 2.85 angstrom.</details>
        <segmentationList/>
        <micrographsFilePattern>data/cryosparc/J1/imported_mrcs/*.mrcs</micrographsFilePattern>
        <pickedParticlesFilePattern>data/cryosparc/J11/P10_J11_passthrough_particles.cs</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/cryosparc/J1</pickedParticlesDirectory>
    </imageSet>
</entry>
