<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12707" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2025-04-15</depositionDate>
            <releaseDate>2025-06-27</releaseDate>
            <updateDate>2025-06-27</updateDate>
        </keyDates>
        <title>Single particle cryo-EM micrographs and particles of OLE RNA dimer</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-6935-518X</authorORCID>
            <firstName>Rachael</firstName>
            <middleName>Christine</middleName>
            <lastName>Kretsch</lastName>
            <organization type="academic">Biophysics Program, Stanford University</organization>
            <townOrCity>Stanford</townOrCity>
            <stateOrProvince>CA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94305</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-8910-3078</authorORCID>
            <firstName>Wah</firstName>
            <lastName>Chiu</lastName>
            <organization type="academic">Division of CryoEM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Menlo Park, CA USA; Department of Bioengineering and James Clark Center, Stanford University, Stanford, CA USA; Department of Microbiology and Immunology, Stanford Universi</organization>
            <townOrCity>Stanford</townOrCity>
            <stateOrProvince>CA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94305</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-7497-0972</authorORCID>
            <firstName>Rhiju</firstName>
            <lastName>Das</lastName>
            <organization type="academic">Howard Hughes Medical Institute, Stanford University, Stanford, CA USA; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA USA</organization>
            <townOrCity>Stanford</townOrCity>
            <stateOrProvince>CA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94305</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-6935-518X">Kretsch RC</author>
            <author authorORCID="0009-0000-6122-2457">Wu Y</author>
            <author authorORCID="0000-0003-2272-7473">Shabalina SA</author>
            <author authorORCID="0009-0006-1911-435X">Lee H</author>
            <author authorORCID="0009-0001-6698-9988">Nye G</author>
            <author authorORCID="0000-0003-3943-8299">Koonin EV</author>
            <author authorORCID="0000-0002-3579-0327">Gao A</author>
            <author authorORCID="0000-0002-8910-3078">Chiu W</author>
            <author authorORCID="0000-0001-7497-0972">Das R</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="GB">437.7</datasetSize>
        <entryDOI>10.6019/EMPIAR-12707</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-48163</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="true">
                    <author authorORCID="0000-0002-6935-518X" order="1">Kretsch RC</author>
                    <author authorORCID="0009-0000-6122-2457" order="2">Wu Y</author>
                    <author authorORCID="0000-0003-2272-7473" order="3">Shabalina SA</author>
                    <author authorORCID="0009-0006-1911-435X" order="4">Lee H</author>
                    <author authorORCID="0009-0001-6698-9988" order="5">Nye G</author>
                    <author authorORCID="0000-0003-3943-8299" order="6">Koonin EV</author>
                    <author authorORCID="0000-0002-3579-0327" order="7">Gao A</author>
                    <author authorORCID="0000-0002-8910-3078" order="8">Chiu W</author>
                    <author authorORCID="0000-0001-7497-0972" order="9">Das R</author>
                    <title>Naturally ornate RNA-only complexes revealed by cryo-EM</title>
                    <journal>bioRxiv</journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country>United States</country>
                    <year>2024</year>
                    <externalReferences type="doi">10.1101/2024.12.08.627333</externalReferences>
                    <details>The micrographs are were collected on a 300 kV Titan Krios with 60 e/A2 total dose. The pixel size for micrographs is 0.954 A and for particle images is 1.431 A.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Aligned and dose-weight averaged micrographs of OLE RNA dimer.</name>
        <directory>/data/ole_particles</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>6324</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>0.954</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>0.954</pixelHeight>
        </dimensions>
        <details>The micrographs are the dose-weighted average by patch motion correction in cryosparc.</details>
        <segmentationList/>
        <micrographsFilePattern>data/ole_particles/S11/motioncorrected/*doseweighted.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Particle images of OLE RNA dimer.</name>
        <directory>/data/ole_particles</directory>
        <category>picked particles - single frame - processed</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>87716</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>360</imageWidth>
            <pixelWidth>0.954</pixelWidth>
            <imageHeight>360</imageHeight>
            <pixelHeight>0.954</pixelHeight>
        </dimensions>
        <details>Particle parameters are from the final C2 reconstruction of OLE. Local motion trajectories are provided in data/ole_particles/J767.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern>data/ole_particles/J1149_particles_exported.cs</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/ole_particles/J1149</pickedParticlesDirectory>
    </imageSet>
</entry>
