REL
2025-05-08
2025-07-01
2025-07-01
CryoEM dataset of Cas12j3 (Casphi3) in complex with crRNA and target DNA after cleavage (postcatalytic state)
0000-0001-6286-2309
Arturo
Carabias del Rey
Novo Nordisk Foundation Center for Protein Research
Blegdamsvej 3B, DK-2200 Copenhagen N
Copenhagen
Hovedstaden
Denmark
2200
0000-0002-8269-3941
Guillermo
Montoya
Novo Nordisk Foundation Center for Protein Research
Blegdamsvej 3B, DK-2200 Copenhagen N
Copenhagen
Hovedstaden
Denmark
2200
Carabias A
Fuglsang A
Temperini P
Pape T
Sofos N
Stella S
Erlendsson S
Montoya G
Novo Nordisk Foundation
NNF0024386, NNF17OC0030788, NNF14CC0001, NNF17SA0030214, NNF18OC0055061
Denmark
5.6
10.6019/EMPIAR-12791
EMDB
molecule
EMD-12827
Carabias A
Fuglsang A
Temperini P
Pape T
Sofos N
Stella S
Erlendsson S
Montoya G
Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3
Nature communications
Nat Commun
1
12
4476
na
2021
English
10.1038/s41467-021-24707-3
34294706
Unaligned multi-frame movies of Cas12j3/crRNA/DNA complex in postcleavage state.
/data/Images-Disc1
micrographs - multiframe
MRC
MRC
4445
40
1
4445
SIGNED 16 BIT INTEGER
4096
0.832
4096
0.832
Movies were collected in a Titan Krios G2 equipped with a TFS Falcon III camera operated at 300 keV in counting mode at a magnification of x 96000 (0.832 A˚ /px) using a total dose of 42 e/A˚ 2. Spherical Aberration: 2.7 mm. Out of the 4445 original micrographs deposited (gain-corrected, non-motion-corrected, 40 fractions), 4393 were used for picking.
Microscope Settings:
-Acceleration Voltage: 300 kV
-Pixel Size: 0.832 Å
-Spherical Aberration: 2.7 mm
-Total Exposure Dose: 42 e/Ų
-Number of Fractions: 40
data/Images-Disc1/GridSquare_*/Data/*s.mrc