REL
2025-05-26
2025-07-01
2025-07-01
CryoEM dataset of Retron-Eco1 E106A filaments
0000-0001-6286-2309
Arturo
Carabias
Novo Nordisk Foundation Center for Protein Research
Blegdamsvej 3B, DK-2200 Copenhagen N
Copenhagen
Hovedstaden
Denmark
2200
0000-0002-8269-3941
Guillermo
Montoya
Novo Nordisk Foundation Center for Protein Research
Blegdamsvej 3B, DK-2200 Copenhagen N
Copenhagen
Hovedstaden
Denmark
2200
Carabias A
Camara-Wilpert S
Mestre MR
Lopéz-Méndez B
Hendriks IA
Zhao R
Pape T
Fuglsang A
Luk SH
Nielsen ML
Pinilla-Redondo R
Montoya G
Lundbeckfonden
R380-2021-1448
Denmark
Novo Nordisk Foundation
NNF14CC0001
Denmark
Novo Nordisk Foundation
NNF0024386
Denmark
Novo Nordisk Foundation
NNF17SA0030214
Denmark
Novo Nordisk Foundation
NNF18OC0055061
Denmark
Novo Nordisk Foundation
NNF23OC0082600
Denmark
European Union (EU)
ER- C_AdG_101096548
Denmark
9.7
10.6019/EMPIAR-12796
Exception
molecule
Carabias A
Camara-Wilpert S
Mestre MR
Lopéz-Méndez B
Hendriks IA
Zhao R
Pape T
Fuglsang A
Luk SH
Nielsen ML
Pinilla-Redondo R
Montoya G
Retron-Eco1 assembles NAD<sup>+</sup>-hydrolyzing filaments that provide immunity against bacteriophages
Molecular cell
Mol Cell
11
84
2185
2202000000000000.0
2024
English
10.1016/j.molcel.2024.05.001
38788717
Unaligned multiframe micrographs of Retron-Eco1 E106A mutant
/data/GM_artcar_acdr0052_Ec86_Filament_20220720_094043/Images-Disc1/GridSquare_19557699/Data
micrographs - multiframe
MRC
MRC
7748
40
1
7748
UNSIGNED 16 BIT INTEGER
4096
0.832
4096
0.832
Movies were collected in a Titan Krios G2 equipped with a TFS Falcon III camera operated at 300 keV in counting mode at a magnification of x 96000 (0.832 A˚ /px) using a total dose of 41 e/A˚ 2. Spherical Aberration: 2.7 mm. Out of the original 7748 micrographs, 7250 were used for particle picking. Micrographs are gain-corrected, non-motion-corrected.
Microscope Settings:
-Acceleration Voltage: 300 kV
-Pixel Size: 0.832 Å
-Spherical Aberration: 2.7 mm
-Total Exposure Dose: 41 e/Ų
-Number of Fractions: 40
The images for every grid square are in different directories following the next architecture:
data/GM_artcar-acdr0052_86_Filament_20220720_094043/Images-Disc1/GridSquare_*/Data
data/GM_artcar_acdr0052_Ec86_Filament_20220720_094043/Images-Disc1/GridSquare_*/Data/*s.mrc