REL
2025-07-15
2025-08-04
2025-08-04
Structure of in-vivo formed alpha-synuclein fibrils purified from a M83+/- mouse brain injected with recombinant 1B fibrils
0000-0001-9168-8450
Lukas
C.L.M.
van den Heuvel
School of Basic Sciences, Institute of Physics, Laboratory of Biological Electron Microscopy, Swiss Federal Institute of Technology (EPFL)
Rte de la Sorge
Lausanne
Vaud
Switzerland
1015
0000-0002-1185-4592
Henning
Stahlberg
School of Basic Sciences, Institute of Physics, Laboratory of Biological Electron Microscopy, Swiss Federal Institute of Technology (EPFL)
Rte de la Sorge
Lausanne
Vaud
Switzerland
1015
0000-0001-8184-5248
François
Ichas
CNRS, Institut des Maladies Neurodégénératives, Université Bordeaux
146 Rue Léo Saignat
Bordeaux
France
F-33000
van den Heuvel L
Burger D
Kashyrina M
de La Seigliere H
Lewis AJ
De Nuccio F
Mohammed I
Verchere J
Feuillie C
Berbon M
Arotcarena M.
Retailleau A
Bezard E
Canron M
Meissner WG
Loquet A
Bousset L
Poujol C
Nilsson KPR
Laferriere F
Baron T
Lofrumento DD
De Giorgi F
Stahlberg H
Ichas F
Swiss National Science Foundation
IC00I0L-231578
Switzerland
Agence Nationale de la Recherche (ANR)
ANR-24-CE93-0003
France
18.1
10.6019/EMPIAR-12890
EMDB
molecule
EMD-54402
Burger D
Kashyrina M
Lewis AJ
De Nuccio F
Mohammed I
de La Seiglière H
van den Heuvel L
Verchère J
Feuillie C
Berbon M
Arotçarena M
Retailleau A
Bezard E
Laferrière F
Loquet A
Bousset L
Baron T
Lofrumento DD
De Giorgi F
Stahlberg H
Ichas F
1.94 Å structure of synthetic α-synuclein fibrils seeding MSA neuropathology
bioRxiv
2024
10.1101/2024.07.01.601498
Unaligned multi-frame micrographs (EER), pre-selected manually for the presence of twisting fibrils.
/data/micrographs/multiframe
micrographs - multiframe
TIFF
EER
41
936
SIGNED BYTE
16384
variable
16384
variable
Micrographs which were used for particle picking in RELION, motion-corrected using RELION's own implementation of MOTIONCOR. Five files are listed for each micrograph: (1) the aligned MRC, (2) the micrograph power spectrum used for CTF correction ("_PS.mrc"), (3) the shifts used in motion correction ("_shifts.eps"), (4) the shifts and header in .star format (".star") and (5) the log file of the motion correction.
data/micrographs/singleframe/Images-Disc1-MotionCorr/FoilHole_*_Data_*_EER.mrc
Extracted particles of manually picked fibrils (box size 800, binning 2, inter-box distance of 14.4A).
/data/particles/Images-Disc1-Extract
picked particles - single frame - unprocessed
MRCS
MRCS
40
1
16 BIT FLOAT
400
1.464
400
1.464
Extracted particles of manually picked fibrils (box size 800, binning 2, inter-box distance of 14.4A), before purification by a series of 2D classification and selection. Two files are listed for each micrograph: (1) the mrcs file with dimensions (N,400,400) where N is the number of particles extracted from that micrograph, and (2) the star file listing the coordinates of each particle ("_extract.star").
data/particles/Images-Disc1-Extract/FoilHole_*_Data_*_EER.mrcs
data/particles/particles.star
data/particles/Images-Disc1-Extract/