REL
2025-08-10
2025-11-04
2025-11-04
Cas1-Cas2/3 heterohexamer bound to foreign dsDNA fragment
0000-0001-9836-3827
William
Henriques
Department of Microbiology and Cell Biology, Montana State University
128 Cooley Laboratory
Bozeman
Montana
United States
59717
0000-0001-9297-5304
Blake
Wiedenheft
Department of Microbiology and Cellular Biology, Montana State University, Bozeman, MT
128 Cooley Laboratory
Bozeman
Montana
United States
59717
Henriques WS
Bowman J
Hall LN
Gauvin CC
Wei H
Kuang H
Zimanyi CM
Eng ET
Santiago-Frangos A
Wiedenheft B
40 frame movies were collected at a 150 ms frame rate and a dose rate of 8.63 e-/Å2/s for a total dose of 51.78 e-/Å2 with a pixel size of 0.833 Å/px over a defocus range of -0.9 to -2.8 um.
Henriques WS
Bowman J
Hall LN
Gauvin CC
Wei H
Kuang H
Zimanyi CM
Eng ET
Santiago-Frangos A
Wiedenheft B
Structures reveal how the Cas1-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci
Structure (London, England : 1993)
Structure
2025
English
10.1016/j.str.2025.09.007
41072406
Unaligned multi-frame micrographs of the Cas1-2/3 heterohexameric complex bound to a fragment of dsDNA
/data/rawdata
micrographs - multiframe
MRC
MRC
2511
40
1
40
SIGNED 16 BIT INTEGER
4096
0.8365
4096
0.8365
40 frame movies were collected at a 150 ms frame rate and a dose rate of 8.63 e-/Å2/s for a total dose of 51.78 e-/Å2 with a pixel size of 0.833 Å/px over a defocus range of -0.9 to -2.8 um.
data/rawdata/n22mar17c*frames.mrc