REL 2025-06-09 2025-11-14 2025-11-14 Cryo-tomography of 5 nm Nanogold labeled ribosomes from intact cells (ExoSloNano) 0000-0003-3494-8302 Lindsey Young University of California, San Diego La Jolla California United States 92093 Elizabeth Villa Howard Hughes Medical Institute, UC San Diego La Jolla California United States 920932 Elizabeth Villa Howard Hughes Medical Institute, UC San Diego La Jolla California United States 92093 Young LN Sherard A Zhou H Shaikh F Hutchings J Riggi M Narasimhan M Riggi M Flaherty WA Rosen MK Giraldez AJ Villa E 41.6 10.6019/EMPIAR-12919 FIB-SEM cell Young LN Sherrard A Zhou H Shaikh F Hutchings J Riggi M Rosen MK Giraldez AJ Villa E ExoSloNano: Multi-Modal Nanogold Tags for identification of Macromolecules in Live Cells & Cryo-Electron Tomograms bioRxiv 2024 10.1101/2024.10.12.617288 Unaligned tilt series associated with 5 nm Halo-Nanogold labeled ribosomes in intact cells /data/frames tilt series MRC TIFF 37 10 1 10 UNSIGNED 16 BIT INTEGER 4096 1.068 5760 1.068
Unaligned tilt series from cryo-FIB-ET to which 5nm Halo-Nanogold has been delivered by ExoSloNano to label ribosomes as a proof of principle.