REL 2025-10-15 2025-11-13 2026-01-20 0000-0003-4247-4303 Ricardo Diogo Righetto Biozentrum, University of Basel Spitalstrasse 41 Basel Switzerland 4056 0000-0002-0941-4387 Benjamin Engel Biozentrum, University of Basel Spitalstrasse 41 Basel Switzerland 4056 McCafferty CL van den Hoek H Müller A Van der Stappen P Righetto RD Stearns T Engel BD National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35GM130286 United States 1.1 10.6019/EMPIAR-13055 EMDB cell EMD-55386 Bertiaux E Louvel V McCafferty CL van den Hoek H Batman U Mukherjee S Bournonville L Mercey O Méan I Righetto RD Müller A Van der Stappen P Buss G Daraspe J Genoud C Stearns T Engel BD Hamel V Guichard P PLoS biology PLoS Biol 12 23 2025 English 10.1371/journal.pbio.3003519 41364719 IsoNet tomograms /data/bin4_isonet reconstructed volumes MRC MRC 72 1 SIGNED BYTE 928 14.08 928 14.08

Tomograms were denoised using IsoNet after running cryo-CARE. One model per tomogram was trained.

cryo-CARE tomograms /data/bin4_cryocare reconstructed volumes MRC MRC 72 1 SIGNED BYTE 928 14.08 928 14.08

Tomograms were denoised using cryo-CARE. One model per tomogram was trained.

CTF-corrected tomograms (phase-flipped) /data/bin4_phaseflip reconstructed volumes MRC MRC 72 1 32 BIT FLOAT 928 14.08 928 14.08

Tomograms reconstructed with CTF correction by phase-flipping in IMOD.

Raw tomograms /data/bin4_raw reconstructed volumes MRC MRC 73 1 32 BIT FLOAT 928 14.08 928 14.08

Raw tomograms reconstructed by filtered backprojection in IMOD.

Tilt series /data/tilt-series tilt series MRC MRC 72 1 32 BIT FLOAT 3712 3.52 3712 3.52

Tilt series processed with Etomo in IMOD. Bad tilts were manually excluded. Corresponding .xf and .tlt files are available for the tomogram reconstruction, as well as the original TS before dose-filtering and the stacks generated from ODD/EVEN raw frames from cryo-CARE denoising.