REL 2025-09-23 2025-11-13 2025-11-13 0000-0001-6653-6682 Mark A Herzik, Jr Department of Chemistry and Biochemistry, University of California, San Diego 9500 Gilman Drive La Jolla California United States 92093 0000-0001-6653-6682 Mark A Herzik, Jr Department of Chemistry and Biochemistry, University of California, San Diego 9500 Gilman Drive La Jolla California United States 92093 0000-0002-4879-9603 Matthew D Daugherty School of Biological Sciences, University of California, San Diego 9500 Gilman Drive, Natural Sciences Building, Room 5315 La Jolla California United States 92093 0000-0001-5854-2388 Kevin D Corbett Department of Cellular and Molecular Medicine, University of California, San Diego 9500 Gilman Drive, CMM East, Room 2058 La Jolla California United States 92093 Cook B Corbett KD Daugherty MD Herzik Jr MA National Institutes of Health (NIH) K22 AI119017 United States National Institutes of Health (NIH) R35 GM133633 United States National Institutes of Health (NIH) R00 HG009530 United States National Institutes of Health (NIH) U24 HG009889 United States National Institutes of Health (NIH) RF1 MH126719 United States National Institutes of Health (NIH) R01 HG011864 United States National Institutes of Health (NIH) R01 HG004659 United States National Institutes of Health (NIH) U24 HG011735 United States National Institutes of Health (NIH) R01 AI188431 United States National Institutes of Health (NIH) R35 GM144121 United States National Institutes of Health (NIH) T32 GM133351 United States Defense Advanced Research Projects Agency (DARPA) HERMES Program HR001124S0025-FP-005 United States Burroughs Wellcome Fund Investigators Pathogenesis of Infectious Disease Program United States 1.6 10.6019/EMPIAR-13082 EMDB molecule EMD-48323 Glasner DR Todd C Cook B D'Urso A Khosla S Estrada E Wagner JD Bartels MD Hung CT Ford P Prych J Hatch KS Yee BA Ego KM Liang Q Holland SR Case JB Corbett KD Diamond MS Lee B Yeo GW Herzik Jr MA Van Nostrand EL Daugherty MD Nature microbiology Nat Microbiol 2025 English 10.1038/s41564-025-02138-w 41093992

CryoEM movies of the IFIT2-IFIT3 heterodimer from Mus musculus. Data were collected on a Titan Krios G3 electron microscope operating at 300 kV and equipped with a Gatan BioContinuum energy filter with a 20-eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.854 Å) on a Gatan K2 detector direct electron detector. Micrographs were acquired in MRC format with a cumulative electron exposure of 65 electrons/Å2 with a defocus range of − 1.0 to − 2.5 µm. Data were collected automatically using EPU with aberration free image shift.

Unaligned multi-frame micrographs of the IFIT2-IFIT3 heterodimer from Mus musculus /data/Movies micrographs - multiframe MRC MRC 2355 50 UNSIGNED BYTE 3838 0.854 3710 0.854

Unaligned multi-frame micrographs of the IFIT2-IFIT3 heterodimer from Mus musculus. Data were collected on a Titan Krios G3 electron microscope operating at 300 kV and equipped with a Gatan BioContinuum energy filter with a 20-eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.854 Å) on a Gatan K2 detector direct electron detector. Micrographs were acquired in MRC format with a cumulative electron exposure of 65 electrons/Å2 with a defocus range of − 1.0 to − 2.5 µm. Data were collected automatically using EPU with aberration free image shift.

Aligned micrographs of the IFIT2-IFIT3 heterodimer from Mus musculus /data/Micrographs micrographs - single frame MRC MRC 2355 1 UNSIGNED BYTE 3838 0.854 3710 0.854

Aligned micrographs of the IFIT2-IFIT3 heterodimer from Mus musculus. Data were collected on a Titan Krios G3 electron microscope operating at 300 kV and equipped with a Gatan BioContinuum energy filter with a 20-eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.854 Å) on a Gatan K2 detector direct electron detector. Micrographs were acquired in MRC format with a cumulative electron exposure of 65 electrons/Å2 with a defocus range of − 1.0 to − 2.5 µm. Data were collected automatically using EPU with aberration free image shift.

Gain file for motion correction of unaligned micrographs of the IFIT2-IFIT3 heterodimer from Mus musculus /data/Gain micrographs - single frame MRC MRC 1 1 UNSIGNED BYTE 3838 0.854 3710 0.854

Gain file for motion correction of unaligned micrographs of the IFIT2-IFIT3 heterodimer from Mus musculus.