REL
2025-11-23
2025-12-22
2025-12-22
Cryo electron ptychography of the Thermoplasma acidophilum 20S proteasome
0000-0002-8451-9947
Xueming
Li
School of Life Sciences, Tsinghua University
Beijing
China
100084
0000-0002-8451-9947
Xueming
Li
School of Life Sciences, Tsinghua University
Beijing
China
100084
Li T
Li S
Yan Z
Li X
National Natural Science Foundation of China (NSFC)
32430056
China
Ministry of Science and Technology (MoST, China)
2024YFA1307302
China
1.8
10.6019/EMPIAR-13137
EMDB
molecule
EMD-67291
EMD-67292
Li T
Li S
Yan Z
Shen Y
Li X
Sampling Mismatch and Correction for Ptychographic Single-Particle Analysis
Diffraction images of T20S proteasome
/data/session1
diffraction images
MRC
MRC
222
1
32 BIT FLOAT
variable
variable
variable
variable
These are the convergent beam electron diffraction (CBED) images of T20S proteasome in EMPAD G1 data format (.raw). Each set of CBED images corresponds to one ptychographic micrograph. Key parameters of each set of CBED images are listed below:
1. Acceleration voltage: 300 kV
2. Convergence semi-angle: ~ 3.69 mrad
3. Defocus: ~ 1.0-2.0 μm
4. Scanning step size: 10.20 Å (nominal value: 11.20 Å)
5. STEM array: 300 × 300
6. CBED image pixel size: 0.005570 1/Å
7. Number of CBED image pixels: 128 × 128
Diffraction images of T20S proteasome
/data/session2
diffraction images
MRC
MRC
166
1
32 BIT FLOAT
variable
variable
variable
variable
These are the convergent beam electron diffraction (CBED) images of T20S proteasome in EMPAD G1 data format (.raw). Each set of CBED images corresponds to one ptychographic micrograph. Key parameters of each set of CBED images are listed below:
1. Acceleration voltage: 300 kV
2. Convergence semi-angle: ~ 3.69 mrad
3. Defocus: ~ 1.0-2.0 μm
4. Scanning step size: 12.25 Å (nominal value: 13.44 Å)
5. STEM array: 250 × 250
6. CBED image pixel size: 0.005570 1/Å
7. Number of CBED image pixels: 128 × 128