The dataset was collected on JEOL CRYO ARM 200 II microscope at 200 kV accelerating voltage, Cs = 1.53 mm, Cc = 1.8 mm.


Folder contents:

20231222_231005.apoF-GFP.4 - non-gain-normalized movies in compressed TIFF format

auxiliary_files - gain reference "231005.apoF-GFP.4_gain.mrc"; CryoSPARC processing workflow "200kv_apoferritin_workflow.json"; Exported CryoSPARC particle stack from the last 3D refinement job "J103_particles_exported.cs"


Related EMDB and PDB entries:

EMD-68251
PDB-22FX


Experiment details:

	○ Mag.: x150k
	○ Pixel size: 0.3056 A/physpix
	○ CLA 100 um
	○ Spot 3
	○ Angle 3
	○ Focus area offset 1.2 um
	○ View mode: x5k, Spot 7, Angle 8, CL3: C0B3
	○ Beam diameter ~950 nm
	○ NO OLA
	○ C3: 8AA8
	○ BA2: 8410  7813
	○ Exp. rate: 3.3 e/physpix/s
	○ Dose rate:  35.335 e/A2/s
	○ Exposure time: 1.51 s
	○ Total dose: 53.356 e/A2
	○ Frame time:  0.0185 s
	○ Frames: 81
	○ Exposure per frame: 0.6587 e/A2/frame
	○ BS-IS acquisition pattern: 3x3x1 in the center of each hole
	○ Data was collected in non-gain-normalized "Counting" mode with CDS, e.g. with 2x hardware binning.
	○ Movie size  ~125 MB
	○ Target defocus -0.5 um with 0.02 um increments between holes.
	○ Total number of micrographs: 13,654 collected in ~56 h; average throughput: ~244 movies/hour.