<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13203" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2026-01-13</depositionDate>
            <releaseDate>2026-03-09</releaseDate>
            <updateDate>2026-03-09</updateDate>
        </keyDates>
        <title>100 kV apoferritin at 1.91 A with DECTRIS SINGLA detector on CRYO ARM 200 II</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-6406-8993</authorORCID>
            <firstName>Radostin</firstName>
            <lastName>Danev</lastName>
            <organization type="academic">The University of Tokyo</organization>
            <townOrCity>Tokyo</townOrCity>
            <country>Japan</country>
            <postOrZipCode>1130033</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-6406-8993</authorORCID>
            <firstName>Radostin</firstName>
            <lastName>Danev</lastName>
            <organization type="academic">The University of Tokyo</organization>
            <townOrCity>Tokyo</townOrCity>
            <country>Japan</country>
            <postOrZipCode>1130033</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-6406-8993">Danev R</author>
            <author authorORCID="0000-0003-0313-2343">Yanagisawa H</author>
            <author authorORCID="0000-0002-5442-7582">Yamashita K</author>
            <author authorORCID="0000-0002-1261-7126">Eisenstein F</author>
            <author authorORCID="0000-0001-7656-8194">Kikkawa M</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Japan Agency for Medical Research and Development (AMED)</fundingBody>
                <code>JP25ama121002j0004</code>
                <country>Japan</country>
            </grantReference>
            <grantReference>
                <fundingBody>Japan Society for the Promotion of Science (JSPS)</fundingBody>
                <code>22H02554</code>
                <country>Japan</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">8.0</datasetSize>
        <entryDOI>10.6019/EMPIAR-13203</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-68204</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="true">
                    <author authorORCID="0000-0001-6406-8993" order="1">Danev R</author>
                    <author authorORCID="0000-0003-0313-2343" order="2">Yanagisawa H</author>
                    <author authorORCID="0000-0002-5442-7582" order="3">Yamashita K</author>
                    <author authorORCID="0000-0002-1261-7126" order="4">Eisenstein F</author>
                    <author authorORCID="0000-0001-7656-8194" order="5">Kikkawa M</author>
                    <title>Atomic resolution cryo-EM at 200 keV</title>
                    <journal>bioRxiv</journal>
                    <journalAbbreviation>bioRxiv</journalAbbreviation>
                    <country>United States</country>
                    <externalReferences type="doi">10.64898/2026.01.09.698548</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Uncounted, unaligned, non-gain-normalized raw movies in HDF5 format</name>
        <directory>/data/HDF5_movies</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>BIG DATA VIEWER HDF5</headerFormat>
        <dataFormat>BIG DATA VIEWER HDF5</dataFormat>
        <numImagesOrTiltSeries>17424</numImagesOrTiltSeries>
        <framesPerImage>13500</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>13500</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>1028</imageWidth>
            <pixelWidth>1.17</pixelWidth>
            <imageHeight>1062</imageHeight>
            <pixelHeight>1.17</pixelHeight>
        </dimensions>
        <details>To process the movies, please contact DECTRIS at support@dectris.cloud to get access to their electron counting software tool that allows converting the HDF5 movies to MRC movies.

The gain reference file is in auxiliary_files/gain.mrc

The CryoSPARC workflow is in auxiliary_files/100kv_apoferritin_workflow.json

The CryoSPARC particle stack from the last 3D refinement is in auxiliary_files/J126_particles_exported.cs

For further details please see the dataset_description.txt file</details>
        <segmentationList/>
        <micrographsFilePattern>data/HDF5_movies/*data*.h5</micrographsFilePattern>
        <pickedParticlesFilePattern>data/auxiliary_files/J126_particles_exported.cs</pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
