REL
2017-05-11
2017-08-16
2020-06-25
40 Degree Tilted Single-Particle CryoEM of Highly Preferred Orientated Influenza Hemagglutinin Trimer
0000-0001-6656-6320
Yong Zi
Tan
New York Structural Biology Center/Columbia University
89 Convent Ave
New York
New York
United States
10027
0000-0002-8124-7472
Dmitry
Lyumkis
Laboratory of Genetics and Helmsley Center for Genomic Medicine, The Salk Institute for Biological Studies
10010 North Torrey Pines Road
La Jolla
California
United States
92037
Tan YZ
Lyumkis D
1.8
10.6019/EMPIAR-10097
EMDB
EMD-8731
Tan YZ
Baldwin PR
Davis JH
Williamson JR
Potter CS
Carragher B
Lyumkis D
Addressing preferred specimen orientation in single-particle cryo-EM through tilting
Nature methods
Nat. Methods
United States
8
14
793
796
2017
English
10.1038/nmeth.4347
28671674
Unaligned raw multi-frame 40 degree tilted micrographs of Influenza Hemagglutinin Trimer
/data/Raw-Frames
micrographs - multiframe
MRC
MRC
668
100
1
100
32 BIT FLOAT
3838
1.31
3710
1.31
Unaligned raw multi-frame 40 degree tilted micrographs of Influenza Hemagglutinin Trimer. Micrographs were collected when tilt angle is set to -40 degrees. The gain normalization reference images (bright and norm) are included for each of the 3 sessions. No dark gain reference images are present because dark images values are always zero in counting mode camera.
Aligned, summed and exposure weighted 40 degree tilted micrographs of Influenza Hemagglutinin Trimer
/data/Dose-Weighted-Micrographs
micrographs - single frame
MRC
MRC
668
1
32 BIT FLOAT
3838
1.31
3710
1.31
Aligned, summed and exposure weighted 40 degree tilted micrographs of Influenza Hemagglutinin Trimer. Micrographs were aligned and summed from the raw frames using MotionCor2, with 5 by 5 patch and exposure weighting.
Equalized particle stack of Influenza Hemagglutinin Trimer from 40 degree tilted micrographs
/data/Particle-Stack
picked particles - multiframe - processed
MRC
MRC
130000
1
32 BIT FLOAT
256
1.31
256
1.31
Equalized particle stack of Influenza Hemagglutinin Trimer from 40 degree tilted micrographs. Particles were extracted from aligned, exposured weighted and summed micrographs. In order to compare these particles with a 40 degree tilted dataset, these particles were equalized based on GCTF-estimated per-particle CTF values so a subset of particles with the same defocus spread would be obtained. This equalized stack of 130,000 is the uploaded stack. A Relion .star metadata file is also provided containing the final refined Euler angles.