REL 2017-05-24 2017-11-28 2021-10-25 0000-0001-5909-701X Jan Ellenberg European Molecular Biology Laboratory Meyerhofstrasse 1 Heidelberg Germany 69117 0000-0001-5909-701X Jan Ellenberg European Molecular Biology Laboratory Meyerhofstrasse 1 Heidelberg Germany 69117 Otsuka S Steyer AM Schorb M Hériché JK Hossain MJ Sethi S Kueblbeck M Schwab Y Beck M Ellenberg J 26.8 10.6019/EMPIAR-10102 FIB-SEM Otsuka S Steyer AM Schorb M Hériché JK Hossain MJ Sethi S Kueblbeck M Schwab Y Beck M Ellenberg J Nature structural & molecular biology Nat Struct Mol Biol 1 25 21 28 2017 English 10.1038/s41594-017-0001-9 29323269

The pixel size in xy was set to be 5 nm and the FIB-milling was done every 8 nm. The acquired raw FIB-SEM images were aligned using TrackEM2 (Cardona et al., PloS one, e38011, 2012) in ImageJ (http://rsbweb.nih.gov/ij/). After alignment, the stack was cropped, gray levels were inverted and the images were smoothened with a mean filter (kernel size: 15 × 15 nm). We used the second half of the cell for segmentation.

FIB-SEM images of a dividing cell at 3.9 min after anaphase onset /data micrographs - multiframe TIFF TIFF 2293 1 UNSIGNED BYTE 4628 5.0 2716 8.0

The pixel size in xy was set to be 5 nm and the FIB-milling was done every 8 nm. The acquired raw FIB-SEM images were aligned using TrackEM2 (Cardona et al., PloS one, e38011, 2012) in ImageJ (http://rsbweb.nih.gov/ij/). After alignment, the stack was cropped, gray levels were inverted and the images were smoothened with a mean filter (kernel size: 15 × 15 nm). We used the second half of the cell for segmentation.