REL
2017-08-25
2017-10-23
2017-10-23
Mixed under-/over-focused data collected by VPP-Cs-corrector coupled EM.
0000-0001-9494-8780
Hong-Wei
Wang
School of Life Sciences, Tsinghua University
Tsinghua University
Beijing
Beijing
China
100084
0000-0001-9494-8780
Hong-Wei
Wang
School of Life Sciences, Tsinghua University
Tsinghua University
Beijing
Beijing
China
100084
Fan X
Wang HW
34.4
10.6019/EMPIAR-10122
EMDB
EMD-6802
Zhao L
Liu C
Zhang JC
Fan K
Yan X
Peng HL
Lei J
Wang HW
Near-Atomic Resolution Structure Determination in Over-Focus with Volta Phase Plate by Cs-Corrected Cryo-EM
Structure (London, England : 1993)
Structure
10
25
1623
1630.e3
2017
English
10.1016/j.str.2017.08.008
28943337
Dose-weighted image from MotionCor2
Dose-weighted image from MotionCor2 for Mixed under-/over-focused data
/data/apoferritin
micrographs - single frame
MRC
MRC
1
1
32 BIT FLOAT
4096
0.88
4096
variable
Micrographs are corrected, summed and dose-weighted by MotionCor2 (from a 33-frame movie with 24 electron per squared Angstrom total dose). run_data.star contains the refine parameters in Relion-2.0. particle coordinates are in the 1st and 2nd column respectively.