CryoET of apoferritin with 0.5 mM TCEP single particle with spot-to-plunge time of 170ms

REL 2018-04-03 2018-04-05 2018-04-05 CryoET of apoferritin with 0.5 mM TCEP single particle with spot-to-plunge time of 170ms 0000-0002-0624-5020 Bridget Carragher New York Structural Biology Center 89 Convent Avenue New York NY United States 10027 0000-0002-0624-5020 Bridget Carragher New York Structural Biology Center 89 Convent Avenue New York NY United States 10027 Noble AJ Wei H Dandey VP Zhang Z Potter CS Carragher B 31.6 10.6019/EMPIAR-10171 EMDB EMD-7627 Noble AJ Wei H Dandey VP Zhang Z Potter CS Carragher B Reducing effects of particle adsorption to the air-water interface in cryoEM BioRxiv English 10.1101/288340 Tilt-series image stacks /data/SerialEM tilt series MRCS MRC 3 1 SIGNED 16 BIT INTEGER 3712 2.34 4984 2.34

Not frame-aligned. .mdoc files included.

Manual particle picking in Dynamo /data/manual_particle_picking particle picking DYNAMO TBL AND OMD FILES DYNAMO TBL AND OMD FILES 1 1 32 BIT FLOAT variable variable variable variable

Binned by 4 manual particle picking of the series0001 tomogram using Dynamo. Manual picking was performed to accurately distinguish between particles adsorbed to the air-water interfaces, non-adsorbed particles, and particles over or adsorbed to carbon.

Appion-Protomo tilt-series alignments /data/protomo_alignments tilt series MRC MRC 3 1 32 BIT FLOAT 3712 2.34 4984 2.34

Includes a full tilt-series alignment run for each tilt-series. To view properly, navigate to the protomo_alignments folder in Appion-Protomo.