<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10176" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2018-03-06</depositionDate>
            <releaseDate>2018-07-27</releaseDate>
            <updateDate>2018-07-27</updateDate>
        </keyDates>
        <title>Single particle cryo-EM dataset of the flexible and variable oligomeric state complex AP-1:Arf1:tetherin-HIV-Nef</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-1717-8134</authorORCID>
            <firstName>Kyle</firstName>
            <middleName>L</middleName>
            <lastName>Morris</lastName>
            <organization type="academic">University of California, Berkeley</organization>
            <street>360 Stanley Hall</street>
            <townOrCity>Berkeley</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94720</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-5054-5445</authorORCID>
            <firstName>Jim</firstName>
            <middleName>H</middleName>
            <lastName>Hurley</lastName>
            <organization type="academic">University of California, Berkeley</organization>
            <street>374D Stanley Hall</street>
            <townOrCity>Berkeley</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94720</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-1717-8134">Morris KL</author>
            <author>Buffalo CZ</author>
            <author authorORCID="0000-0001-5054-5445">Hurley JH</author>
        </authorsList>
        <datasetSize units="GB">115.4</datasetSize>
        <entryDOI>10.6019/EMPIAR-10176</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-7458</emdbEntry>
            <emdbEntry>EMD-7457</emdbEntry>
            <emdbEntry>EMD-7456</emdbEntry>
            <emdbEntry>EMD-7455</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Morris KL</author>
                    <author order="2">Buffalo CZ</author>
                    <author order="3">Stürzel CM</author>
                    <author order="4">Heusinger E</author>
                    <author order="5">Kirchhoff F</author>
                    <author order="6">Ren X</author>
                    <author order="7">Hurley JH</author>
                    <title>HIV-1 Nefs Are Cargo-Sensitive AP-1 Trimerization Switches in Tetherin Downregulation</title>
                    <journal>Cell</journal>
                    <journalAbbreviation>Cell</journalAbbreviation>
                    <country></country>
                    <issue>3</issue>
                    <volume>174</volume>
                    <firstPage>659</firstPage>
                    <lastPage>671.e14</lastPage>
                    <year>2018</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1016/j.cell.2018.07.004</externalReferences>
                    <externalReferences type="pubmed">30053425</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Dose weighted particle stack of AP1:Arf1:tetherin-HIV-Nef</name>
        <directory>/data/dataset_all_209816_ptcls</directory>
        <category>picked particles - multiframe - unprocessed</category>
        <headerFormat>MRCS</headerFormat>
        <dataFormat>MRCS</dataFormat>
        <numImagesOrTiltSeries>209816</numImagesOrTiltSeries>
        <framesPerImage>38</framesPerImage>
        <frameRange>
            <frameRangeMin>3</frameRangeMin>
            <frameRangeMax>38</frameRangeMax>
        </frameRange>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>384</imageWidth>
            <pixelWidth>1.067</pixelWidth>
            <imageHeight>384</imageHeight>
            <pixelHeight>1.067</pixelHeight>
        </dimensions>
        <details>Picked particles after 2D class averaging in Relion to clean the dataset. 209,816 particles represent the AP1:Arf1:tetherin-Nef dataset before 3D classification of the multiple oligomeric and flexible states of the complex.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
